XB-CLONE-4351061
Clone Name: IMAGE:7026864
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Gene:
wsb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785131 [+]
taattaatcccccccccccaagaaggtttccagctgagagggctgcaggggcttccgttcgctaagcgttccctatgggacggagacgggtcagcgaccc
acacggattgcggtgttgatggagtaacagtggggcaccgagtcagatgtcttgtttggtgtgaagactttcccgtcagcgccctgacccgcacccacat
cacacccactccctgtaatatgagtgaggcagcacggagctggcactgtatagcccgaagctgctcattctccagtatgaaaggctccctgttgtttatc
ccctccatagggggaaataaaagcgcttatgtatagtggccaacgcgtcggatcctttgttattgcctttatttggggatttgcaagtcgctgctgcacc
tgcgaccaggacgagttgtcgccaccaagctcctttcctggctggactgagtgactctcatctggcactaattggtggatttggtagcggcggtgcagtc
aagcagaatggaagacgttcctgtcctgctgggagaactgcgtcctggcagggaacagtcatatgactggaagtcaagttgcgaaacctggagcgttgcg
ttttctcctgatgggtgttggtttgcttggtcccaaggacatggcattgtcaagctcctgccctggccactgggaggaagaacgtttacggcccccagcc
ttttgctttctaaaaccaatgtatctaggatcccagatatttaaaggacgacgaaattcctgagggaaaaagacactgggactgggggtccaaataattt
tggggg
3' EST: CF785130 [+]ttttttttttttttttcatgtcagtatcactttaagattgattcaagctgggagggaaaaacaccttgtagggcaaaaattgcatagtaaattgtccaag
aggggaaaaaaaaaagtaaatgcaattgaaataattgaattctgccagaacccagtatgttctagatagctcacatctactaatctgaatagaacaggtg
atggtgactttatcatttacagaccttaccacaatgtacttctctttatcaggtattaatatatttgctttttgccaatatgttggctcttgttccatgg
aggtaccagtaaggtgcactttagccaggagtacatctacgtccactgtgggggttatttgtgaaatcagtgtggtgtcaaatacttttaaataaggcgg
cttgaaggtcccctattgctgctccttacacaaagaggctataaataagtccgataagtaagaaaatccttcatttttcctggaataggtaaagcaagga
cttgataagtggtaaggaagcaacgtagtgccttgcgacacaagtgccggagtgaggacaggaccttcggggaagtccagaaccgcacgtggccatctct
attgcctgttgcaagaactcccccatgtggcaaaaaagtacagcacaaaccatttgtgactggtgcaacagacacaggatcttgtagctctagagaccat
aagtctcagaaatctgtcatctgccaagctggccaaatagagcccctctggggagaaaacgacggacctccgggaagcttacctggggttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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