XB-CLONE-4351058
Clone Name: IMAGE:7026863
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Gene:
snrpd3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077072 [+]
CGTTTCCTTTCGCTGTGCATTGTGGGGCAGGCGGGACGCCATTAGAACGCAGAACAGAGCCACGCTAGTTAATCTCCATCAGCTCTCAATATGTCTATCG
GTGTACCAATTAAAGTCCTTCATGAGGCAGAAGGCCACATTGTGACATGTGAAACTAACACCGGAGAGGTATACAGGGGGAAATTGATTGAAGCAGAGGA
TAACATGAACTGCCAAATGTCAAACATCACTGTAACGTACAGGGATGGCAGAGTGGCACAACTAGAACAGGTCTACATCAGAGGCAGCAAAATTCGTTTT
CTCATCCTCCCAGACATGTTGAAAAATGCTCCCATGTTAAAGAGTATGAAGAATAAAAACCAGGGTTCTGGTGCCGGCAGAGGAAAAGCAGCTATTCTCA
AGGCCCAAGTGGCAGCGAGAGGAAGAGGCCGCGGGATGGGACGTGGGAATATCTTCCAAAAACGGCGATAACCAGAATTGGTTTTGTATTTTTAATGTGG
TGTTTTGATATGTTCCTGAATGTTTCCATTTTTGTTTAGTTTTTTTTTTCCTTTTGATTAATAAAACTGTTCATGAACCAAAAAAAAAAAAAAAAAAAAA
A
5' EST: CF785133 [+]aattaatccccccccccccccgtttcctttcgctgtgcattgtggggcaggcgggacgccattagaacgcagaacagagccacgctagttaatctccatc
agctctcaatatgtctatcggtgtaccaattaaagtccttcatgaggcagaaggccacattgtgacatgtgaaactaacaccggagaggtatacaggggg
aaattgattgaagcagaggataacatgaactgccaaatgtcaaacatcactgtaacgtacagggatggcagagtggcacaactagaacaggtctacatca
gaggcagcaaaattcgttttctcatcctcccagacatgttgaaaaatgctcccatgttaaagagtatgaagaataaaaaccagggttctggtgccggcag
aggaaaagcagctattctcaaggcccaagtggcagcgagaggaagaggccgcgggatgggacgtgggaatatcttccaaaaacggcgataaccagaattg
gttttgtatttttaatgtggtgttttgatatgttcctgaatgtttccatttttgtttagttttttttttccttttgattaataaaactgttcatgaacca
aaaaaaaaaaaaaaaaaaaaaggatcccctagttctaaagcggccgcccaccgcgggtggacctttggccgggggccccaacttgttttttttgcagctt
taaaagggttaccaaataaaggcaatagccctcccaaattttcccaaaataaagcatttttttttcactgccattcctaatttggggggttgggccccaa
cctctcatcaaggggtatctttaataatggcccggggatcgga
3' EST: CF785132 [+]tttttttttttttttttttttggttcatgaacagttttattaatcaaaaggaaaaaaaaaactaaacaaaaatggaaacattcaggaacatatcaaaaca
ccacattaaaaatacaaaaccaattctggttatcgccgtttttggaagatattcccacgtcccatcccgcggcctcttcctctcgctgccacttgggcct
tgagaatagctgcttttcctctgccggcaccagaaccctggtttttattcttcatactctttaacatgggagcatttttcaacatgtctgggaggatgag
aaaacgaattttgctgcctctgatgtagacctgttctagttgtgccactctgccatccctgtacgttacagtgatgtttgacatttggcagttcatgtta
tcctctgcttcaatcaatttccccctgtatacctctccggtgttagtttcacatgtcacaatgtggccttctgcctcatgaaggactttaattggtacac
cgatagacatattgagagctgatggagattaactagcgtggctctgttctgcgttctaatggcgtcccgcctgccccacaatgcacagcgaaaggaaacg
gngggggggggggggattaattac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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