XB-CLONE-4351055
Clone Name: IMAGE:7026862
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Gene:
morf4l1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084908 [+]
GGTGCGGTGACGTGCGGGTCCGCGCGGTGAGTAGATGCGGCGGATCCGTTCCCGGGAGCCGCGGAGTTTATAAATGGCGCCGAAGCAGGACCCAAGGCCT
AAGTTCCAAGAGGGTGAGCGTGTCTTGTGTTTTCATGGTCCTCTGCTTTATGAAGCTAAGTGTGTGAAAGTTGCCATTAAGGACAAACAAGTGAAGTACT
TCATTCACTACAGCGGATGGAATAAGAACTGGGATGAGTGGGTACCAGAAAGCAGAGTACTCAAATACGTGGACACCAACTTACAGAAACAAAAAGAACT
TCAGAAGGCCAATCAGGATCAGTATGTAGAGGGGAAGATGAGAGCAGCAGCACCAGGAAAGAAGACTGCTGCCCTTCAACAGAAGAATGTAGAAGTCAAA
ACAAAGAAGAATAAACAGAAAGGCCCTGGAAGTGGAGAGGGCAGCAGCACAAGTGACATTCCGCAGCCACCACGGAAAAAACGGGCACGGATAGATCCCA
CTGTGGAAAGTGAGGAGACTTTTATGAACAGAGTTGAAGTGAAGGTAAAGATTCCAGAGGAGCTAAAACCTTGGCTTGTGGATGACTGGGATTTAATCAC
ACGACAGAAACAGCTGTTTAATCTTCCAGCAAAGAAAAATGTGGAAACTGTTTTGGAGGAGTATGCTACGTATAAAAAATCTCGGGGAAATACCGATAAC
AAGGAATATGCTGTAAATGAAGTTGTGGCAGGGATCCAAGAATACTTTAATGTAATGCTTGGCACACAGCTGCTATACAAATTTGAACGTCCCCAGTATG
CAGACATTCTGGCAGACCATCCAGATGCCCCCATGTCTCAAGTGTATGGAGCACCACACCTTCTGCGTCTATTTGTCCGAATTGGAGCCATGTTGTCTTA
TACTCCCCTCGATGAAAAGAGTCTGGCACTGCTTCTCAACTATCTACATGATTTCTTAAAGTATTTAGCCAAAAATTCCTCCTTGCTCTTTAGTGCCAGT
GACTATGAAGTGGCGCCCCCAGAATACCACAGGAAAGCAGTATAGTTTGATTTAAGAACACCACAGACAGAACGGCTTTTGAGCATTGTTGTGACTGCTC
TTATGACTTTTAAAACCCCCAAATTAAGAAAAAAAAAAAAAAA
5' EST: CF785135 [+]taattaatcccccccccccccggtgcggtgacgtgcgggtccgcgcggtgagtagatgcggcggatccgttcccgggagccgcggagtttataaatggcg
ccgaagcaggacccaaggcctaagttccaagagggtgagcgtgtcttgtgttttcatggtcctctgctttatgaagctaagtgtgtgaaagttgccatta
aggacaaacaagtgaagtacttcattcactacagcggatggaataagaactgggatgagtgggtaccagaaagcagagtactcaaatacgtggacaccaa
cttacagaaacaaaaagaacttcagaaggccaatcaggatcagtatgtagaggggaagatgagagcagcagcaccaggaaagaagactgctgcccttcaa
cagaagaatgtagaagtcaaaacaaagaagaataaacagaaaggccctggaagtggagagggcagcagcacaagtgacattccgcagccaccacggaaaa
aacgggcacggatagatcccactgtggaaagtgaggagacttttatgaacagagttgaagtgaaggtaaagattccagaggagctaaaaccttggcttgt
ggatgactgggatttaatcacacgacagaaacagctgtttaatcttccagcaaagaaaatgtggaaactgttttggaagagtatgctacgtataaaaaaa
tctcgggggaaataccgataacaagggaatatgcctgtaaatgaaatttgtgggcagggattccaagaataactttaatggtaaatgctttggccaacac
agctgtgctttaacaaaattttgaaacgctcccccct
3' EST: CF785134 [+]ttttttttttttttcttaatttgggggttttaaaagtcataagagcagtcacaacaatgctcaaaagccgttctgtctgtggtgttcttaaatcaaacta
tactgctttcctgtggtattctgggggcgccacttcatagtcactggcactaaagagcaaggaggaatttttggctaaatactttaagaaatcatgtaga
tagttgagaagcagtgccagactcttttcatcgaggggagtataagacaacatggctccaattcggacaaatagacgcagaaggtgtggtgctccataca
cttgagacatgggggcatctggatggtctgccagaatgtctgcatactggggacgttcaaatttgtatagcagctgtgtgccaagcattacattaaagta
ttcttggatccctgccacaacttcatttacagcatattccttgttatcggtatttccccgagattttttatacgtagcatactcctccaaaacagtttcc
acatttttctttgctggaagattaaacagctgtttctgtcgtgtgattaaatcccagtcatccacaagccaaggttttagctcctctggaatctttacct
tcacttcaactctgttcataaaagtctcctcactttccacagtgggatctatccgtgcccgttttttccgtggtggctgcggaatgtcacttgtgctgct
gccctctccactttccagggcctttcngtttattcctcctttgtttggacttctacatttcctctgttgaaaggcaacaatccttctttcctggtgctg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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