XB-CLONE-4351044
Clone Name: IMAGE:7026858
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785142 [+]
taattaatcccccccccccccccgctccccagtcgttgccggaagagaacggttgtacctacaagtagaaagtaaaaatgtctatcaagaagatccttgc
acgtgaaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcctccact
ggcatttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtggctaatgcagttgagcacatcaataaaaccattgcac
ctgcactgataggcaagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatttggtgc
taatgcactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctgggaaccca
gaggtcatccttcctgtaccggcatttaatgtgataaacgggggttctcatgctggaaacaagctggctatgcaagaattcatgatcttgcctgtgggtg
cagacacctttaaagaggccatgcgcataggaacagaggtgtatcataacctaaagaaagtcatcaaggctaaatatggaccagatgccactaatggtgg
gtgatgaaaggaagcttttgctcccaaaaatcttggagaaataagggaaacctctgggagctttcctgaaaacctggccaatcaaacaagggctggggct
tacccccagattaaaagtt
3' EST: CF785141 [+]ttttttttttttttcaccttcaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaactgcaataggatgagaacacaa
gactacaaagttgacatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactgaatttggaataatcctaagca
gcaaagcttttaaactggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaaattaaatgagggctcagcagt
gacatgtaagacctcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccatttagttgaaaacaggttttct
gaaatttcttccagcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgctcagatctgcatggagcacct
gtcttgatctgtccagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactgaccatcacaccccagccatttg
actgagccagcttgcacgcctgcagggattctgtcactgtgccgatctggttgaccttcagaagcagacagttacaagacttctcatccacagccttggc
aatacgtttagggttggtcactgtcaagtcatccccaactacctggatattcgaaaaagccgttaaattttttccagggcttccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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