XB-CLONE-4351033
Clone Name: IMAGE:7026854
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Gene:
phgdh
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785149 [+]
taattaatcccccccccccccccaaaggatttgtgagatagagagagagactgcagggacccggctgcacagagacagcaagatatctgcagggagacag
acagacagacatgtctctatcagacatccgccgggtgcttatcagtgacagcctggcaccagcctgtgcccaaaccctacgcacccagggggggatcgag
gtgacggagagcccgggactgacacatcagcagctcctagggcagatacaggactatgatggtctcattgtacgttcagccaccaaagtcacagcagaag
tcctgactgcggggtccaggctgaaactggtgggacgagcggggacaggagtggacaatgtggacgttgagtgcgccaccaagaaaggcatcatcgtaat
gaacaccccaacagggaacagcatcagtgcagcagaactcacctgcggactcattctaagcctgtccaggcaaattccacaggctgctgaatccatgaga
gctggaaaatgggatcggaaaaagttcatgggatcagaactgtatgggaaaacagtaggaatccttggccttggtcgaattgggaaagaggtggctatca
gaatgcagtcattccagatgcggaccatcggttacgaccccatcatcccccctgaggtgaccgctcagtttggagttgagcagttttcactggaagaaat
ctgggagcagtgcgattatatcacagtacatactcccctcctaccctctactacagggctgataaatgacgcccgcattcgcaaaatgcaaaacggggaa
gtaaaagttatat
3' EST: CF785148 [+]tggtttggtctcaggcgagaatgctctgaatagagccggtgcgttgacagctcccaccagcgctcggtccttcaccaggtcgacaatttgttgagcgatc
tcttccccgcagcgattctgtgcttcctccgtgctggccccgaggtgggggaggctaatgaccagggggtgctccactaaggctcgatctctgggaggct
cctctacaaatacatccaacccagctccgccacactgcccactctgcaacgcccttagcagtgccgcctcatcgatgatgccccctctagcgcaatttat
aacttttactccccgtttgcactttgcgaatgcggcgtcatttatcagccctgtagtagagggtaggaggggagtatgtactgtgatataatcgcactgc
tcccagatttcttccagttgaaactgctcaactccaaactgagcggtcacctcaggggggatgatggggtcgtaaccgatggtccgcatctggaatgact
gcattctgatagccacctctttcccaattcgaccaaggccaaggattcctactgttttcccatacagttctgatcccatgaactttttccgatcccattt
tccagctctcatggattcagcagcctgtggaatttgcctggacaggcttagaatgagtccgcaggtgagttctgctgcactgatgctgttccctgttggg
gtgttcattacgatgatgcctttcttggtggcgcactcaacgtccacattgtccactcctgtccccgctcgttccaccagttttcagcctggaacccgcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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