XB-CLONE-4350955
Clone Name: IMAGE:7026824
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Gene:
tram1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785199 [+]
taattaatcccccccccccccggtgtcgccggtgtgtgtggcacatagaagtctcggccctgtatgcgccatcctgtcaccatgggcatccgtaagaaga
acagcaagaccccgccggtgctgagccatgagttcattatccagaaccatgcggacatcgtctcctgcctggccatggtcttccttcttgggcttatgtt
cgagattacagcaaagatggctgtcatgtttgtcacgcttcagtataatgtcaccattccagtagaaggggtgcttggagaacccgcttcgctgtaccat
tatggcatcaaagatatggccactgtatttttttacatgctggtggcaataatactacatgcagttatccaggaatacatattagataaaatcaaccggc
gtatgcacttttctaagacaaaacacagcaagtttaacgagtctggacagctaagtgcattttacctgttctcttgcatttggggagcgagcattattgt
ctctgagaattatttttctgatccaataagcttgtggaaagggtatcctcacacatactttccattccagatgaagttcttctacatttcccagctggct
tattggtttcatgcttttccagagctctacttccaggaaactaaaaaggaagacattcccaggcagcttgtctacattggtttgtacctattccatatta
taggaagcttatgtttctcaacctcaatcgccctgggtctggtattactaggttttgcattaactttggtagaaattcctgttttccatgtt
3' EST: CF785198 [+]tttttttttttttttcgtgaagctttgcctttagcagaggttgctttcttcctttgggaggagggttgaggagaggaatgctctctccaccgtctcagct
ggaaattaatgaacttccacatcataaatgcctgggttatgcaaatagatgccagcacactgattctgatggccaaaatgttgaaatttccattactcaa
atccaactcctgattttcagcccgtgcaagtccaaagccaacagtaagaacagaaaggataagagtgaggaggcgcccaaggacaaagagaacagcccac
aaagtaaaccctttctggtatttctcattgctgaagtaaaacagacgtgacatgtgaaacaggaattctacaaagtaatgcaaaactagtaataccagac
ccaggcgattgaggttgagaacataagctcctataatatggaataggtacaaaccaatgtagacaagctgcctgggaatgtcttcctttttagttttctg
gaagtagagctctggaaaagcatgaaaccaataagccagctgggaaatgtagaagaacttcatctggaatggaaagtatgtgtgaggataccctttccac
aagcttattggatcagaaaaataattctcagagacaataatgctcgctccccaaatgcaagagaacaggtaaaatgcacttagctgtccagactcgttaa
acttgctgtgttttgtcttagaaaagtgcatacgccggttgattttatctaatatgtattcctggataactgcatgtagtattattgccaccagcatgta
a
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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