XB-CLONE-4350915
Clone Name: IMAGE:7026807
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Gene:
mybph
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785224
[+]
taattaatcccccccccccccccgttgcaattgctgagagcagagtcagaaccatgccagcaaaggctccaattaaaaaatcccctgcagctaaggctgc
tccagctaagccacaggcagctcctgtacctgtacctgagccagtagaggaagtccctactcctgtgccagaagcccctgctcctattcctgagcctgtt
gagaataccgttcaagaggcacctgcaccacccacagaacccccagcaccagaggcagaagtttctgtcccagcaccagaaacacctacaccagctgcag
aaccagaaaaggctcctgaagtagttgaaccacccccacctgaaccaaaagaagaaccaccaagtgagcctacagatttaacagtggatgaaattgatga
cacttcaatatcccttaaatggaaagctccagagaaggaaggaactactgccctggatggatacttactggaataccaaaaagaaggcagtcaagaatgg
attgctgtgaacaaagagccaagtgtttcaactcgatacaaaattcagaatttgaatactggtgataagataactgtttgtgttanagcagttagcaaag
caggaagcagtgctccaagagcacttgaaaattttgttcaaattcgagaaaattgtggatcgtcccaaaatccgtcttccaaggtatttgcgtcaaacat
ttgtgaaaacatgtaggagaaacagtgaaactagtgatcccgtttcaaggggtaagccaagaacccccagggtcacctggactaaaaaatggaacagccc
tctgggacccctaa
3' EST: CF785223
[+]tttttttttttttttcttttttttttttttttttttttttttgggctgaataaattctttattataagtcatatgaaatacaataaatacaggacatcat
ttgtgttgagtaaactgtatgttattttgtgccttcattaatttcattcagcaatcttctatttgttatttatgtcctgtttactggggcactctaacat
ccagcctgcaatctacacttgcttcccctaatgagttgattgctttacaggtgtaaactccaccatcaaaaggacagggcttgcgaatttcaagagagca
gactccctgattaacaaggcaccgaaatttagggtcctctctaatttccatttgattctttagccacacaattctaggctttgggcttccacggacacaa
cagaacagtttagtgctatatccagttgttgttgctctattagcaagcggctgggtaaattttggtggctctgcaaaatctcgttccttgtactctgggg
gcttataaatgattcctgttttcttaataacagcagcatcttttattactgcagcctttttcactagtccccacaaagttttcaaaaaatactctgaagt
aaaaaagaaattcccatggatcaggttccgaaaaaaagggccattaattcttggccaaaaaagcctccctgtaaaaataaaaccaatttcccccaaagtt
tttcctgggccccgccttttttggccccacagggggaaacccccgggaaaaaaactcccaaaaatatttcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: