XB-CLONE-4350909
Clone Name: IMAGE:7026805
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Gene:
lrrc8c
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080460
[+]
CCCGAGGACAGGGAGGACAAGCCGGAGTGTGGGAAGAGGAAGGCAACTGCGCAGCCACAGCATCATCCCGCTATTCCCCTGGCACAGGAAAGCCGGGCAT
GACTGCACTGACACTGCTGCTCGGAGCACGACCAGCCTGGATCAGGAAACATGATTCCAGTGACGGAGTTCCGACAGTTTTCCGAGCAGCAGCCAGCATT
CCGTGTTCTCAAGCCATGGTGGGACGTGTTCACGGACTATCTGTCTGTGGCAATGCTGATGATCGGAGTCTTTGGTTGCACATTACAGGTCATGCAAGGC
AAGATAATATGCCTGCCAAGAGTGCAGCCCATTCACAACATTTCAGTCTACAATGTTTCAAATTCTAATCTGCCACTTAATACACCAAAGCCAATAACAC
CTGGGACAGTGGAGATGAAGGGGCTGAAAACAGACTTGGACCTTCAGCAGTACAGTTTTATTAACCAGATGTGTTACGAGCGTGCTCTGCATTGGTACGC
CAAGTACTTTCCCTACCTTGTTCTGATTCATACTTTAATTTTCATGTTGTGTAGCAACTTTTGGTTTAAATTTCCTGGCTCAAGTTCGAAAATTGAGCAT
TTTATATCAATCTTGGGGAAATGTTTTGATTCTCCTTGGACAACCAGAGCCTTGTGCAAAGTATCTGGAGAGGAGCCAGAAGAGAAGGATAACAGAAAGA
ATAATCTGAATAAGTCTAACATGAACCTGTCACCACTGGAGAGTCCCTTGGTGAAGACCCAGTCTCTGAAGTCAATACCTGAGAAGTTTATTGTGGATAA
AGCTGCTGCAGGTGCTCTGGATAAAAAGGAAGGAGAGCAAGCAAAAGCCTTGTTTGAGAAGGTGAAAAAGTTTCGCATTCATGTTGAAGAAGGAGATCTC
CTTTATGTCATGTATGTCCGCCAAACCATTCTTAAAGTTCTGAAATTTCTATTTATCATTGGATACAATAGTGCTCTTGTGTCAGAAGTACGGTTTACTG
TAGAGTGTAATGTTGACATACAGGACATGACAGGATATAATCATTTCTCTTGCAATCATACAATGGCTCATCTGTTCTCTAAGCTTTCCTTTTGTTACCT
CTGCTTTGTGGGTGTTTATGGCCTCACTTGCCTTTATACTCTGTATTGGCTATTCTATCGCTCTTTAAAGGAATATTCATTTGAATATGTTCGTTTGGAG
ACTGGCATCAATGATATCCCAGATGTGAAAAATGATTTTGCCTTCATGTTCCACATGATAGATCAGTATGACCCTTTGTATTCTAAACGCTTTGCTGTGT
TCCTCTCTGAAGCTAGCGAAAACAAGCTGAAGCAACTCAACTTAAACAATGAGTGGACAGCAGAAAAGCTGAGGCAAAGACTTCAGACCAATGCATCCAA
CCGGCTGGAGTTACAGTTGTTTATGCTCTCTGGCCTTCCAGACACTATATTTGAGATAACAGAGCTACAGTCCTTAAAGCTGGAAATTATAAATAATGTT
ATGATTCCAGCAACTATTGCTCAGCTGGACAATCTGCAAGAGCTATCATTGTGTCAGTGTTCTGTAAAGATTCACAGTGCAGCACTGGCATTCCTAAAGG
AGAATCTAAAGATTCTGAGAGTTAAGTTTGATGATGTTCGAGAGCTTCCACCTTGGATGTATGGGTTACGAAACCTGGAGGAATTATATCTGATTGGATC
TTTAAGCCCTGATGTATCAAAAAACATCACACTTGACTCATTCCGTGAACTGAAGAGCCTTAAACACTTATTTATAAAGAGTAATTTATCCAAAATCCCT
CAAGGTGCTGTTGATGTTTCTGGCCATCTTCAGAAACTCTGCATTCATAATGATGGAACTAAGCTGGTCATGCTGAACAACTTGAAAAAGATGGTCAACC
TGACAGAGTTGGACCTCATACACTGTGACTTGGATCACATACATCACTCGATTTTCAGTTTGTTAGCACTGGAAGAACTGAATTTAAAGGAAAATAATCT
CAAGTCTATTCAAGAAATAGTTAGTTTTCAATACCTGCGCAAACTTACAATCTTAAAATTGTGGTACAACAGCATTACACATATTCCAGTTGAAATAAAA
AAACTCACCAGCTTGGAGAGGCTTTACTTTAGCCACAATAAAATAGAGGTTCTCCCATCTCAGCTCTTCCTATGCCACAAACTCAGATACTTAGATTTGT
CATACAACGACATAAGATTTATTCCACCAGAAATTGGAGTAATGCAGTCTTTACAGTATTTTTCAATTTCTTGCAACAAAGTGGAGAGCGTTCCTGATGA
ACTGTACTTCTGCAAGAAGCTGATAACATTAAAAAAAAAAAAAAAA
5' EST: CF785228
[+]taattaatccccccccccccccccgaggacagggaggacaagccggagtgtgggaagaggaaggcaactgcgcagccacagcatcatcccgctattcccc
tggcacaggaaagccgggcatgactgcactgacactgctgctcggagcacgaccagcctggatcaggaaacatgattccagtgacggagttccgacagtt
ttccgagcagcagccagcattccgtgttctcaagccatggtgggacgtgttcacggactatctgtctgtggcaatgctgatgatcggagtctttggttgc
acattacaggtcatgcaaggcaagataatatgcctgccaagagtgcagcccattcacaacatttcagtctacaatgtttcaaattctaatctgccactta
atacaccaaagccaataacacctgggacagtggagatgaaggggctgaaaacagacttggaccttcagcagtacagttttattaaccagatgtgttacga
gcgtgctctgcattggtacgccaagtactttccctaccttgttctgattcatacttttaattttcctgttgtgtagcaacttttggtttaaatttcctgg
ctcaagttcgaaaaattgagcattttatatcattcttgggggaaatgttttggattctccttggaaaaccagaacccttgtggcaaagttatctgggaga
aggaacccagaagaagaagggttaacccgaaaaggaattattcttgaaataagttcttaacattggaaaccctggtcccacccactgtggaaaaaacctc
cccttggggtgtgaaagaacccccaaagttcttccttgaaat
3' EST: CF785227
[+]tttttttttttttttaatgttatcagcttcttgcagaagtacagttcatcaggaacgctctccactttgttgcaagaaattgaaaaatactgtaaagact
gcattactccaatttctggtggaataaatcttatgtcgttgtatgacaaatctaagtatctgagtttgtggcataggaagagctgagatgggagaacctc
tattttattgtggctaaagtaaagcctctccaagctggtgagtttttttatttcaactggaatatgtgtaatgctgttgtaccacaattttaagattgta
agtttgcgcaggtattgaaaactaactatttcttgaatagacttgagattattttcctttaaattcagttcttccagtgctaacaaactgaaaatcgagt
gatgtatgtgatccaagtcacagtgtatgaggtccaactctgtcaggttgaccatctttttcaagttgttcagcatgaccagcttagttccatcattatg
aatgcagagtttctgaagatggccagaaacatcaacagcaccttgagggattttggataaattactctttataaataagtgtttaaggctcttcagttca
cggaatgagtcaagtgtgatgttttttgatacatcagggcttaaagatccaatcagatataattccctcaggtttcgtaacccatacatccaaggtggaa
gctctcgaacatcatcaaacttaactctcagaatctttagattctccttttaggaatgccagtgctgcactgtgaaatctttacagaacactggacacaa
atgataggctccttgccagatttgtcccagcttgaggcaataat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: