XB-CLONE-4350902
Clone Name: IMAGE:7026801
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Gene:
krt12.5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785232 [+]
taattaatcccccccccccccgacattctgttttgtgcagactgagttgtcaggcattctcttccaataacagtcaccatgtctctgcgttctagctctt
cttattctgtacagagcaaaagtgtctctggaggtggtggttttggcagcggctatggggctagctttggtggaggaagctttagtggaggaagctatgg
tggcagcagctttggtggtggctttggagcaggctttggtggtggtgcagcttctgggttttccgctagttccgcaggtggttttggagcagcatccgct
agttccagcttcagcagctttggcggaagcgacaagcaaaccatgcagaacctcaatgaccgtcttgctgcctaccttgagaaagtgagagccttggaag
ctgccaatgctgacttggaactgaagatccgcgagtggtatgagaagcaaagaacctctggcattggagcaggggctaaggatttcagcaaatactttga
tatcatcactgatttgaggagcaagatcctttttgccaccattgataactcaagagttgttctgcanattgacaatgccaaacttgctgctgatgacttc
cgtctgaagtttgagaaagagctggctcttcgccagagcgtggaggctgatatcaatggcctgcgtaaagtcctgaatgaacctgacctggctagaagtg
acctggaaaagcagatcgaaaacttgaatgaaaaaacttggcctaccctgaaaaaaaaaacctttgaaaaaggaaaatgaactttttgctataaaaccaa
gtc
3' EST: CF785231 [+]tttttttttttttttccaaagaactggcttggaaagctttattgcatcttgaaatcaacagaaatttccattttgtgagtgaagatttcagagatgaatc
tttatttgacgttctcttcgacttcctcaactcttgatgagacaacctttccatcgaccacttcttccacaatggtctttacttttctggtttttgatgg
atcttttttggagctggaagatgattgtgatgtgattacttgcactgatgaactggaagaactcaattctccctccagcaggcgcctgtatgtttcaatc
tccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgacgttccatgtcggacctgatctgcagaagttgttcctctaggcttc
tgatactgagctggatggtttgaagctgggcaccatagcggccatctgtttctgccagagagtcttcaagagattttttcattgccagttgagattgcaa
ttcaatttccaagctctgaaggctgcgtctgaggtcggatatttcgctcttgcttgactgcactagttccacgcctggttaaaatctctttcttcagctc
accactctttctggttgaaccacagctctgcatcccctctgttcttctcagccagtgtttcatagtcggctctcatggtattccgaatgttggccagatc
tgggccctgggacagcgtcctttttcaacgtccacttggccggggagccatggtcttaaaaagggcccaccccccccttcaggggttcttctta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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