XB-CLONE-4350875
Clone Name: IMAGE:7026023
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785249 [+]
taattaatcccccccccccccgatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagagaa
aggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacagaga
cggcatcattgactaggaagacctgagggacacattcgcagctatgggccgcctgaacatgaacaatgaaaagttagaagaaatggtcaaagatgcatct
gggctaatcaacttcaccgtcttcctgaccatgtgcggcgagaagctcaaggtggctgatccttaagacgcaatcactggcccacttcaaggtgctgtga
gcctgaaagtttcggattcatcaagagtgcagttcccttgaaggaactcctgtacgtcacgaatgtgaacacaattgagtcgcgagagggaagaacgagc
aactgtgatcagaagagattatccccacctctttatgagtaccgtaagacaccccttgactcctcttctcggtataaaattaaaagttttatggtgacga
tccggaggcctgcccggcgtttagcgctcctcgtctccatacaaccggctcctagtttcatgccttggtgtgcgcnatcgtaggtgcatagtgaacagcc
ttatctaatgaatgttttcacacatggttcggcanngcatactgcaattatcatcgactccgcatatagacgtaagaaaacgtcaagagtcccaagaata
ttatacctacgatcccgcctgataagatgtgttttgataatacggatcttgcggattacgtatcacatatgatagaagaaacaaaatagagtccagagta
actgagatgtgattaggtgacgcgcagatcgcattaatgcgtataaatagctcgaactgtgtgattacagacccgcggtcgcaatcctatctctactcgc
tgctgctaatatgatctatccatcatgtgtcgagcacgcaccactggttggagagagatgatcacagctctgatggagtagatatgcggcgcgcaatgag
tagatactactcgccactcacagatcgatctg
3' EST: CF785248 [+]tttttttttttttttgaataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagggaggg
taaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttgtgtct
ctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactgggcctgt
ttattcctggtctttgtcctctccgtgggtgatgacataacagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccacatgttc
ttgatctcctctggggtgaatctgtcacactgtgtggaaaggagttcctcaaggaactgcttcttgatggatccttttggcctcagggatcaaccacctt
ggaatgcccccagtgaataacgtccttccgggatcagccaccccttgaagccttctcgcccaaaaaatgggtccgggaaaaaacggtgaaaatttgattg
ggggcccgaatgccctcctttggaccatcttcattcgaaacttcattcatttctttaaagtttcaggggggggccccataaacctgccgaaatggaggtg
cccctccaaggacctttccctagggtccaaagaaatggcacggacctccttgtttcagggaatacaaatccacagggtgggagagacgcccctccccttt
gaacaccacccttggggaatttctgtggggtatatggggtgcacaaaactattggggagaacaacacacctctatgggagatagaaatctcacacacctt
tn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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