XB-CLONE-4350870
Clone Name: IMAGE:7026020
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Gene:
alad
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080497
[+]
CGCAGAAGTTGTTGGGCACTGGGAGAGCGTGAAAATATGCAAGTCAGCGCCATCCTACACAGCGGGTATTTCCACCCTGTTCTGCGGGCCTGGCAGTGCT
CTGCTACCAGCCTCGATGCCAACAACCTCATGTACCCCATCTTCATCACGGATAACCCGGATGCCATAGAGGAGATTCCCAGCCTGCCCGGCCAAGCCAG
GTACGGGGTGAACCAACTGGAGGGGTTGCTGCGCCCCCTAGTGGACAATGGGCTGAAGTGCGTGCTGATCTTCGGGGTCCCAAGCAAAGTCATTAAGGAC
GAGCGGGGCTCCGCGGCAGACGCGCCCGACACGCCGGCCATATTGGCCATACAGAGGATTCGGGAGAAGTTCCCCCAGCTGCTCATTGCCTGTGACGTGT
GCCTTTGTCCCTATACCTCCCACGGCCACTGCGGGATCCTACGGGAAGATGGCAGCCTACAGAACGAGAGCAGTTGCCAGAGACTGGCAGAGGTGGCCCT
TGCTTATGCCCGTGCAGGCTGTCACATCGTGGCGCCGTCGGACATGATGGACGGGCGAATCGGTGCCATCAAGCAGGCGCTGGTCTCCAACGACCTGGGA
AACAAGGTGTCCGTCATGAGTTACAGCGCCAAGTTTGCCTCCTGTTTCTATGGGCCCTTCAGGGACGCCGCGCAGTCCAAACCGGCCTTTGGTGATCGGA
AATGTTACCAGCTCCCCCCCGGCGCCAGAGGCTTGGCCATCCGGGCAGTGGACCGGGATGTGCGGGAAGGGGCAGATATGCTGATGGTGAAGCCCGGGAT
GCCCTACCTGGACCTGGTCAGAGAAGTGAAGGAGAAGCACCCTGCTCTCCCATTGGCTGTGTACCACGTGTCGGGGGAGTACGCCATGCTGTGGCACGGG
GCGCAGGCCAACGCTTTTGACCTCAAGGTGGCCGTGCTGGAAGCAATGACGGGATTCCGGAGAGCCGGAGCCGATATCATCATCACGTACTACACCCCCC
AACTCCTCCAGTGGATAAAGGAACGATAAAGCTGCTCCCCAAATACCCCCCCCCCCCCCCCCCGATGATGTTTGGGGCAATTCCTCACTGCATGGATTTC
CTACAGTGACCCCCGCACTGCCATAGGCCAACCATTTGCTTTAATCCCACGCCCACCATAAACATGGCCGCTCCCTGTAGAAGGGTCTGAGTGGGCAGGA
GTGACCCACAATGCAACTGACCCTTTATTATTCACCTCCCTCTGCCCCACTGCTGCCCCCTGCAGGCCTCTGGTAGAACTGGGGCCAGTAATGGGGCTTA
TTCTGTGTTTCCATCACATTGTTGGACCCATTATAAAGCCGCCTGTACAGCAGCCCAATGAGAATCCGCCTTCTGGTCACATGATCGGTTCCCGCTCTCC
TTGTGCCCAAATTTCAAGACCAAATAAACCCAATTTTTCGTTAAAAAAAAAAAAAAAA
5' EST: CF785251
[+]taattaatccccccccccccccgcagaagttgttgggcactgggagagcgtgaaaatatgcaagtcagcgccatcctacacagcgggtatttccaccctg
ttctgcgggcctggcagtgctctgctaccagcctcgatgccaacaacctcatgtaccccatcttcatcacggataacccggatgccatagaggagattcc
cagcctgcccggccaagccaggtacggggtgaaccaactggaggggttgctgcgccccctagtggacaatgggctgaagtgcgtgctgatcttcggggtc
ccaagcaaagtcattaaggacgagcggggctccgcggcagacgcgcccgacacgccggccatattggccatacagaggattcgggagaagttcccccagc
tgctcattgcctgtgacgtgtgcctttgtccctatacctcccacggccactgcgggatcctacgggaagatggcagcctacagaacgagagcagttgcca
gagactggcagaggtggcccttgcttatgcccgtgcaggctgtcacatcgtggcgccgtcggacatgatggacgggcgaatcggtgccatcaagcaggcg
ctggtctccaacgacctgngaaacaaggtgtccgtcatgagttacagcgccaagtttgcctcctgtttctatgggcccttcagggacgccgcgcagtcca
aaccggcctttgggtgatcggaaatgttaccagctccccccccggcgccagaaggcttggccattccgggcaatgggaccgggatgtgtcggggaggggg
gcagaatatgctggatggg
3' EST: CF785252
[+]tttttttttttttttaacgaaaaattgggtttatttggtcttgaaatttgggcacaaggagagcgggaaccgatcatgtgaccagaaggcggattctcat
tgggctgctgtacaggcggctttataatgggtccaacaatgtgatggaaacacagaataagccccattactggccccagttctaccagaggcctgcaggg
ggcagcagtggggcagagggaggtgaataataaagggtcagttgcattgtgggtcactcctgcccactcagacccttctacagggagcggccatgtttat
ggtgggcgtgggattaaagcaaatggttggcctatggcagtgcgggggtcactgtaggaaatccatgcagtgaggaattgccccaaacatcatcgggggg
ggggggggggggggggtattttn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: