XB-CLONE-4350867
Clone Name: IMAGE:7026019
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Gene:
pcbp3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785254
[+]
taattaatcccccccccccccagaccagcagaggcagcagcagaagccgcagctcccgccagcccctcccgccgtcgccattcctcgcccgcccggctct
ctccttccctccctccccggggccccttgcctccggaccgttccttcctccccattcctcccttgttttcctaccccctttccccattttcttaatcgct
ctcacttttcccgcccttccaccttacggcggtcacctcttgtcctttgtaactccgcagtcttgtctcatcctgtgaccaaagatttgactgctttgta
cagcttctgacaatagccctgatccaacagaactagactcatcatggacaccggtgtgattgaagggggcctgaatgttacactcaccatcagattgctt
atgcatgggaaggaagttggaagcattattggaaagaagggggaatctgttaaaaagatgcgtgaagagagcggtgcccggataaacatctcagaaggga
attgtccagaaaggataattaccctggctggtcctacgaacgcaatctttaaagccttctctatgataattgataaactggaagaggatatcagcagctc
catgaccaacagcactgcatctagcaaacctccggttactttgcgtctggtggttccagcaagtcagtgtggatccctcattggcaaaggtggctgcaaa
ataaaggaaaatagagaaagtacaggagctcaggtacaagtggctggggacattgctgcctatttctacagaacgagcaatcccccattgctgggcaatt
ccgcaagtcaatttatagaa
3' EST: CF785253
[+]tttttttttttttttatttttttcaagttccagtccgtctttatttacttgttttctgaaaaaatcctgacattacagaactaaattgaaatgcatttat
taccccactctttacaatgtgacatttttaaccttaaaaaaaaaaaatttgatagggtacaggacaaactttttataaaactaaaatctgtatcagcagg
ttttaacaactggaaggggaaaaattaaaaatattctcaaaaaaaaattatgaatgagtggaagagggaaagcataaggaaacagattgcaaactagtta
aaacctgaaaagaggctgtttgcagggatgtgcagactgacagtgagctaaacagaagagatgtcagaagaggtctaactgcttcccatgccaccagctt
ctgaggaaagcctgacattaataaggtactgggccaaactaatgctggctgtggatccagttatggtcacctggcgatcatttgaaccctccacagggtt
agctatttttatctgagccccagacatctgacgaatttcatttattttagctccctgtcgtcccatgatacaaccgatgagatcatttgggatgggtgag
ttcatgaaaaaatcctctgaactgaagcttcaatcccccccccttaattcagtgttgccccctatgggacatttggaaaaggaaaactgcctgcattggc
taattggtggcaccttggtcaaaatcaggctgaagaaaggccatactgtccccgggaatggaaaagcccccctggaaaatcccccgcatccaagggagac
ccccccctgtgggggaacccttccccctccctcacaggtcagtccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: