XB-CLONE-4350862
Clone Name: IMAGE:7026017
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Gene:
psap
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785257 [+]
taattaatcccccccccccccttttttttttttttttttgtataagcagtgacttcatttttgtctggttctccaaaacgtttttctccctcttataacc
tgaaaaaaagttcttaggttggtctaatctagaacttttgtcacataatgtgcatttagggaccgatttatcaaagcgtggaattggagctcatcacaga
aactccccactcttcattcctatagaatttttataggcatatttatcaaatgatcagctctaactttcactttacaaatatgctctaaaaatcccatagg
aatgaatagagagcatagattgatgaattttactctggtactttcataaatctgccccttagtcttttaacagggtgcccggcctgtataataagacatt
tcccaagggaagtttatgtgggtcttgattctgcaaagccataaacccctctgatatggattaaaagcagccagggtgcctcagagcggtactcttaggg
caatgccacacagaccatttgtcaccaaatgccccaaagtacccacgcattgctgcccattacaactgcaaccagtatgtcagtcaaatcacatggaaat
gcttgaacttttgcacaattaatttactgacaattgtgttttggcaacaatccggggtatttttggggcaatttggtttgcccatgcctgggtgggggtg
cccactgccttaataaacgcaccatggattttgcaaggtaataccctatttgaatttggggttaaaaaacattttgtttaattaaaccgttttgaaaatt
gttaaaaattttgagacaaaaaagtgggtcctgagacccaaatggagaat
3' EST: CF785256 [+]tttttttttttttttaaaggttttaaagtattttattgtcaattacagcagctttaaagagaaaaaaaaaaaacacatccatgaaacctcttaccctaat
ttattcatctagttccctataaatgtaagttcttatattattcctataaaaggtgcagtctcaggaaagaagggcagccttgatatggaagtgcaataca
agattatgaatagagcaaggcgagcatggtaaccacctacgggaaggctgcagttttatgccaggacatctcagctgctgaaagatcagtgtttgctaca
gttatctcacaaccatgattaattcattagtcagaacatttgttcaaattaaaatctaaacgttaataacagatgtcttaaccaaatcaataggtataca
tgcaaatcatgctgcgttattaagcagtggcaccccaccagcatggggcaacaaattgccccaaaatacccggattgttgccaaacacaatttggtcagt
aaaaaaattgtgcaaaagttcaagcatttccatgtgatttgactgacatactggcttgcagtttgtaatgggcagcaatgcgtgggtactttggggcatt
tgggggacaaatgggcctgtgggggcattgcccaaaaagtaccgccttggaggcaccccgggcggcttttaaacccataaccagagagggtttatggctt
ttttcaaattcaaaaccccacttaaactttcccttgggaaaaagttcttttttaaaagggggcggggcccccctgtttaaaaaaactaaggggggcaaaa
tttattta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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