XB-CLONE-4350859
Clone Name: IMAGE:7026016
|
Gene:
aldob
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785258 [+]
taattaatccccccccccccctcgtcacacctaggagtttctactggcgacttctctcacagcagtaagtacagtaaaattaagcacaatggccccggta
ttcccagcactaactgcagagcagaagaaggagctgtctgagattgcccagcgtattgtggcaccaggcaaaggaatccttgcagcagatgagtctgtgg
ggactatgggaagccgcctcaagcgcatcaatgtggagaacatagaagagaacagacgtctctatcgagagcttcttttcactgcagatccctctataaa
agacagcattggaggtgtcattcttttccatgaaactctctaccagaagtctcgagatggagttctttttcccaaaatcctcaaggatcttggcattgtt
gtaggaatcaaggtagataaaggcactgctcctttagctggaactgatggagagaccacaacacagggtttggatgggcttgcagaaagatgtgctcagt
ataagaaggatggcgcagactttgcaaagtggcgttgtgtgctgaagatctcggattctgcaccatcaagtcttgctatctatgagaatgctaatgtttt
ggctcgatatgccagtatctgccagcagaatggacttgtgccaattgtggaaccagaaatcctgtctgatgggtcacatgatctccaaaggtgtcagtac
gagactgaaaaggtcctgtccgctgtgtatgctgccctaattcagcaccatgtttatctgggaagaactctatttaaaaccaaatatgggtcacaggggg
ggcccagtcttgtttcacaaaaatatacccc
3' EST: CF785259 [+]tttttttttttttttgtatgtaaagatcaattttaatgtggcagaaatacatntttggaataatacaagaagtaaaaaggtcaaactgatgtgattttgt
tgcacactaaatattatttatgaatttgtatacagtatttatctcttgctcaaatgttggaactactttgagtacaaagagggctgaaataaaattgaaa
tatacagattttggcccataccagtaaaaacctatggtacttaaacaattggcatacaaataatctcattctttagaaaatactatttggaatgttggta
aatctgtcattagggcatggattggagactggtcaccaaatgcagattttggtattttgtgcttttaacaactggaatagatcacagacaggctgtttaa
taggtgtaactaggctgaaacagagactgagatgaagcagaatctgaagatccagatggaacatattttccaagagttgccaaaccattaatctttgccc
tcttaacaaaggcttcctgggcagctttctcattctcactcttcccaacccaggcagagagtgctgatgcctgtagagcacgaccgtatgagaaagacac
acgccatggccgattaagactgcagcaattcatagcattcaggttaagggaggcctcttcttcactttgacctcctgagagggagcagatacctggaact
gcaggaaggactgtacgtcttaaaacaatgacagtggccatggcaacatcttgtgggggtatattttttgtgaacaagactgggcccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: