XB-CLONE-4350852
Clone Name: IMAGE:7026013
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Gene:
fhit
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077654 [+]
CCGAAAAAATCAGATTTCTTTTCTGTATGAGGAAATGAGCATTGCTGAAATAATTTGGTGATATTCCTGGGAGGAAAGGGAGTGCTGCTGTCAGAGTCCT
TTATAAAGAAACACTCCTAGGGATTTAGCCAAGCATTTAGCTAAAGCTTTGCAAGGAAGGAAAGTTCCTGTTGCTGCATTTTGCAATGTCGTTAAGGTTT
GGCCAGCACCTTATTAAACCATCCGTTATTTTCTTTAGATCTGAACTCTCTTTTGCTCTGGTGAACAGAAAGCCAGTTGTACCAGGTCATGTACTAGTCT
GTCCACTGCGTCCCGCAAACCGCTTTCGAGAACTAAGGCCAGAAGAAGTCAGTGACCTGTTTACAACTGTCCAGAAAGTGGCCAGCGTAGTGGAAACCCA
TTTTGGTGGTAGCTCCCTTACTATCTCTATCCAAGATGGCCCAGAAGCAGGACAAACCGTTCAGCACGTCCACGTGCACATCCTGCCACGGAAAGCAGGA
GATTTTAAAAGGAATGATAAGATTTATGAGGAGCTGCAGGACCATGACAGCAAAGGGCAGGATACTCCAGATAAATGGAGATCTGAGGAAGAGATGGAAA
AAGAGGCAGCTGCACTCAGGGAATGCTTCTAGTTGAGCCATTCAGGTGCTCATTGGATGTTTTTTTTTCTTTTCTCGGAGTCTATTCATATAACAAGGGA
TGCAGCGATCGTTAGTTAGCAAATAATTTAAATAGTACAGTAGATTCTGGCAAAAACTGAACTAAAAATAAACTTTGTAAAAGCCAAAAAAAAAAAAAAA
A
5' EST: CF785263 [+]taattaatcccccccccccccccgaaaaaatcagatttcttttctgtatgaggaaatgagcattgctgaaataatttggtgatattcctgggaggaaagg
gagtgctgctgtcagagtcctttataaagaaacactcctagggatttagccaagcatttagctaaagctttgcaaggaaggaaagttcctgttgctgcat
tttgcaatgtcgttaaggtttggccagcaccttattaaaccatccgttattttctttagatctgaactctcttttgctctggtgaacagaaagccagttg
taccaggtcatgtactagtctgtccactgcgtcccgcaaaccgctttcgagaactaaggccagaagaagtcagtgacctgtttacaactgtccagaaagt
ggccagcgtagtggaaacccattttggtggtagctcccttactatctctatccaagatggcccagaagcaggacaaaccgttcagcacgtccacgtgcac
atcctgccacggaaagcaggagattttaaaaggaatgataagatttatgaggagctgcaggaccatgacagcaaagggcaggatactccagataaatgga
gatctgaggaagagatggaaaaagaggcagctgcactcagggaatgcttctagttgagccattcaggtgctcattggatgtttttttttcttttctcgga
gtctattcatataacaaggggatgcacgcgatcgttaatttagcaaataattttaaataagtaccagtagattcctgggccaaaaactggaactaaaaaa
taaacctttttgtaaaaaccacaaaaa
3' EST: CF785262 [+]tttttttttttttttggcttttacaaagtttatttttagttcagtttttgccagaatctactgtactatttaaattatttgctaactaacgatcgctgca
tcccttgttatatgaatagactccgagaaaagaaaaaaaaacatccaatgagcacctgaatggctcaactagaagcattccctgagtgcagctgcctctt
tttccatctcttcctcagatctccatttatctggagtatcctgccctttgctgtcatggtcctgcagctcctcataaatcttatcattccttttaaaatc
tcctgctttccgtggcaggatgtgcacgtggacgtgctgaacggtttgtcctgcttctgggccatcttggatagagatagtaagggagctaccaccaaaa
tgggtttccactacgctggccactttctggacagttgtaaacaggtcactgacttcttctggccttagttctcgaaagcggtttgcgggacgcagtggac
agactagtacatgacctggtacaactggctttctgttcaccagaacaaaagagagttcaaatcttaagaaaataacggatggtttaataaggtgctggcc
aaacccttacgaccttggaaaatgcaccaaccggaactttccttccttggaaagcttttacctaatgcttggctaaatccctaaggagtgtttttttttt
aaaggactcttaaaagccgcacttccttttcctccccggaaaatcccccaaattttttccgcaaggcccattttcctcgtaccgaaaaaaaaatctgatt
tttttcggggggggggggggggggaattatattaactcccgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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