XB-CLONE-4350816
Clone Name: IMAGE:7025999
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Gene:
ilf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785286
[+]
taattaatccccccccccccccgccttcccgcgtgtgaaacccgcccccgacgatagctcattcagcgagtcactgctcaaacgaaaccaagatctggcc
cccagcacagccgagcaggcctcaatcctttctttggtcaccaaaatcaacaacgttatcgacaatttgatcgtagccccgggaaactttgaagtgcaaa
ttgaggaggtgcgacaggtgggctcctacaagaaagggaccatgagcacaggccataatgttgcagacttggtcgtcatcctaaagatccttcccacact
ggaagccgtttctgctttgggcattaaggtggtcgagaccctaaggacgcaggatccagctgaagttctcacaatgctgacaaacgagactggctttgag
atcagctcagcagacgccacggtgaagatccttatcacgactgtgccgcccaatctgcgcaagctggaccccgagttgcatttggacatcaaagttctgc
agagcgcgctggccgccatcaggcacgcacggtggttcgaggagaacgcctcccattctacagtgaaggttttaatccggttgttgaaagactttaggag
tcggtttcctggatttgagcctctgactccctgggatcctggacctcttggggcattaatgccgtcatgaacaaccccccccaaggcagcccgctgggca
ctccatggtggtcctaaaaaaacgccggccctaaaaaaatgcttcgccccccccgggcccttttttcctctcccccgggcttcaggtttggggaattcac
attggaaccccccttgggtggaaaac
3' EST: CF785285
[+]tttttttttttttttggaggtgtcgaaataaaaatttattgctggcaaaaaaaaaaaaaaatacaatgtatccctacagtataaaacaagtcacatgaca
agctaagaacgaatgaaaataaaacaggaactcgtggcatttggtccacaaacagacccaatgtgttcggcgccggcagagggggatccagaccaactga
aatctttaaataagtatcactcctgtgtctccatgctctcctcctcttccgccccctctgggatatcctggttctcatcctcctctccctccttcctctc
ggggggcttctcataggccttctctgaaggggtcacgatgactccgtcccaggtagacatttccgtggcaagagcgctggcatctccctccaggcccaat
atcttccggaagcccccgtgggagagaatacggaccaacgtctgggcagtgtagcaaaccatgtcctgctgctccagagtcatgactgtatgaaccctaa
agttgccactttcacaggggtcagtgatgccaactgagccggggaggaaaaggccggcggcgagcatctgtaggcagcgtttgtaggacacattgagtgc
cagcggctgcctgggtggggttgttcatgacggcataatgccccaagaggtccaggatccaggggagtcagaggctcaaatccaggaaaccgactcctca
agtctttcaacaaccggattaaaaccttcactgtagaaatgggaggcgttctccntcaaacaccgtgncgtgccctgaggcggccagcgcgctca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: