XB-CLONE-4350811
Clone Name: IMAGE:7025997
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785289
[+]
taattaatcccccccccccccgagacacacagggcccaagaaaggactaaccacattaccagaggagagggaaccttgacatcagaccagacacccagca
gagcctacaggagaggaacttcaaacatgaactgaacccaggctatcctgagaaacccaatgatttaatcacagaagagaccttagaggtgatggcactt
gggaagaactgtcaccaaggtttttaaaaagcaagtttcgttgacaattcgttcatcttttcagagacaacgttttctggagattctgacctaagtgatc
aacacacgaaagcgaagcaaaaagagaaatagtgtttcaagggaagcagaaaaaacgctcagtgtgtttactcagcggcattctaggcgtattacctcag
caaccttcttggagtgttaaatcagcatccttctctacgtgattactcagcaacatggtatcggatactcaaatgcttgtgttgcttttcatgatatatt
tatatctatattttttggaaaaagaaaggcaagggagacctgctgctagaaagcattggatacatccttttcacaaatcagcgtctaacaaaaggactgc
tttcaggtattggactctaaacttacgctgccggcccgaaaaaaattcccaaaaaacttttgcaggaatggtcaacccaactaagcctttgaatggaaac
tggctggacccattttttaaaagccacgggcccgtggtcttcgggaaaccccaaagacagttgttgaatggaagggggattcccccccggaaggtctctt
aagaagaggggggggcggccccacgcccagccggagtgtgggataccactggtgaagcccggagc
3' EST: CF785288
[+]tcaacgtttgggtacgagacaggcctggctttaaaatggtcagcagttcatcaaagctagttgttgacattctgaaatgtttttggaattttttcgggca
gcagcgtagcttagagtacaatacatgaaacagtccttttgttagacgctgatttgtgaaaggatgtatccaatgctttctagcagcaggtctcccttgc
ctttctttttccaaaaaatatagatataaatatatcatgaaaagcaacacaagcatttgagtatccgataccatgttgctgagtaatcacgtagagaagg
atgctgatttaacactccaagaaggttgctgaggtaatacgcctagaatgccgctgagtaaacacactgagcgttttttctgcttcccttgaaacactat
ttctctttttgcttcgctttcgtgtgttgatcacttaggtcagaatctccagaaaacgttgtctctganaagatgaacgaattgtcaacgaaacttgctt
tttaaaaaccttggtgacagttcttcccaagtgccatcacctctaaggtctcttctgtgattaaatcattgggtttctcaggatagcctgggttcagttc
atgtttgaagttcctctcctgtangctctgctgggtgtctggtctgatgtcaaggttccctctcctctgggtatgtgggtagtcctttcctgggccctgt
gtgtctcgggggggggggggaataatttaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: