XB-CLONE-4350805
Clone Name: IMAGE:7025995
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Gene:
ccnb1.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785293 [+]
taattaatcccccccccccccgaggtttgtagtgaatcagttgccgttaccagtttggaaaaatgtcgctgcgtatcaccagaaacatgatggcaaatgc
agaaaacaatgtgaaaaccactgtagctggaaagagggttgttgcaaccaaaccagttttgaggcctcgtacagctttgggagacataggaaacaaggcc
gagctaaaagttccagcaaaaaaggaactgaagccagcagtaaaagctatcaagaaggtaaaacctgtggacaaagtgttggagcccctgaaagttagag
aagagaatgtttgccccaaacctgctcaggttgaacctagctcaccaagcccaatggaaacatctggttgcctccctgatgagctctgccaggccttctc
tgatgtcttgattcaagttaaagatgttgatgctgatgatgatggcaatccaatgctgtgcagtgaatatgtgaaggacatttactgttaactgaggagc
ctggaaaaagcacaagcagtcagacaaaactaccctacatggacaggaaaatcacagggaaacaatgcgtgccataattggatttgacggggctgggtcc
aagaggccaaatggaaattttccttctgcctgccaggagaaataattgtttccgtgacttgtttgggcaataatttgatatgccatttcctgctagggaa
cattcccagttttcccaataaaaacccaacttgggaaacttttggttggggggtccaaaaaagcaatggattccccttgggcgtgtccaaaacaactgga
agaaaatatggtgtcaccccttccaataaaaattttagaagaaaatgttttacaattttggagaaaccgggaaattcctcacacatatactacataagag
3' EST: CF785292 [+]tttttttttttttttgggcaaatgttaaaatgagctttattaaaaacacaaagtaaaacaaatacactatttacagaagcacatggtgccaaacaatcca
gttataattcacaccagtccatcacatgagtgggcgggccaattccacaacaacatctgatctcagctgggggatcatgctgatcttcatttgtttgctg
ctggcatatttgttcttaacagtcaggtgtttggtcagtcccttgttcaccttaatgatgttcttggccatatgttgcataacagggacaagatcatctt
caaagtaagccatataatgatgcattgttggtgtccattcacccgtattcaagattttaagagacaagcaagaggcagcagctgctatttgggaaggcga
gtaatgcaccatatcataatccaccattacaagttccattaaatatttagccaaactgtgctgttcagcagtcacctctccaatcttagaagctctccga
agaaagtgcagaggtaatggtcggcctattgcaaacttaagcacccgaagcaccttcatttccatgtccctaatttgagcctttgtgtatgtgtgatcag
ttacaaatgtaaagtctccgatttctggtgggtacatctcttcatatttagcagcaaggaacatagctgtgacccccacaagctgcaagttggtttttaa
gaaactggatggtcctgcanaaagcgatcaattatgcccaacagtcatgaaacatagtcttctgcagcaaaacgaaatttcattttgcaactg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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