XB-CLONE-4350796
Clone Name: IMAGE:7025992
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Gene:
eno1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785298
[+]
taattaatccccccccccccccccccgattctccccagtcattgccggaagagaacggttgtacctacaagtagaaagtaaaaatgtctatcaagaagat
ccttgcacgtgaaatttttgactctcgtggtaacccaacagtggaggttgacctgtacacatgcaaaggtctcttccgtgctgctgttccaagcggagcc
tccactggcatttatgaagcactagaactccgggacaatgataagactcgctacatgggaaagggtgtcggaagagctgtcaaatatataaatgagtttc
tgggacctgcattatgcacccagaatctaagtgttgtggagcaggaaaagatcgacaacctcatgattgaaatggatgggactgagaataaatccaaatt
tggtgctaatgcactcttgggagtgtccctagcagtgtgtaaagctggagctgcagagaaaggagtcccactgtaccgtcatatcgctgacctggctggg
aacccagaggtcatccttcctgtaccggcatttaatgtgataaaagggggttctcatgctggaaaacaacctggctatgcaagaattcatgaatttggcc
tgtggggggcagacaccttttaaggaggcccatgccccttaggaacaaaaggtgtatcataacctaaaagaatgtcatcaaaggctaaaatatgggacaa
agaagcccacttaatgttggggtgaataaaaggaaggtttttgcttccccaatattcttgggagaaataagggaaagcccctgggaaacttttctggaaa
aactgcccatctaaa
3' EST: CF785299
[+]ttttttttttttttctttttttttttttttttttcaccttcaaaagagtttattgcctcttcgtgtctagagtgtgacaaaccattatctagtctgaaac
tgcaataggatgagaacacaagactacaaagttgacatgaccaggaccactgccacaatagaaaagacaaggactacatgcaggcaccagtgtgacactg
aatttggaataatcctaagcagcaaagcttttaaactggaaagacagcaaggttatgctgggagaggctgtaacatctaacattgctggaagtgcctgaa
attaaatgagggctcagcagtgacatgtaagacctcctgtagtctctcctaggtgcacagctactacatcagttgggtgagagcctaaggatgcctccat
ttagttgaaaacaggttttctgaaatttcttccagcaaagcgggccttgctgccaagctcttcttcaatcctgagcaattggttgtattttgcaagtcgc
tcagatctgcatggagcacctgtcttgatctgtccagtgcggagaccaaccaccaggtcagcaatgaaagtatcttctgtctccccagagcgatgactga
ccatcacacccccgccatttgactgagccagcttggccctggatattccaagatgcagtaaaattttttccagggctttccaagtcattcctgattcaaa
ggggatcttccaatgggaaacccacctgggaaaaattctttggaacaaagcctccattgtaaaaaatttacaccccagtcttccttctagggggaaaata
tgtgttctttttttagaggggtccttccaggggggaaactttgaaaattccccaaaggttaataattgttccccgcgttccacggggtgaaaaan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: