XB-CLONE-4350793
Clone Name: IMAGE:7025991
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Gene:
darmin
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785301
[+]
taattaatccctcccccccccccggccagtaacaagctgaacactccatcacagggaaacaatatacttcagctatgttccgctggttcctcatatttct
gttaacatgtcaagttctgttatcccctgtgccaaaggatggggtttttcagtatattgatgctcatcaagatgaatttatccagagattaaaggactgg
gtggcaatagaaagtgactctagtgacccatcaaagagagacttgcttaacaaaatgatggaacttacaaaggactttatcctcaaactgggtggaaatg
ttgagatggcagaaattggcgagcaagagctgtcatctgtggagaggattccactgcctccagttatcctagcagagctaggaaatgacaaaagcaaacc
aacggtctgtttctatggacacatggatgttcagccagcaaagcaaacagatgggtggttaacagatccttatactgttgtggagaaagatggtaatctg
tatggaagaggtacctcagatgacaaaggacaagtgcttgctctgctgcatgcactggaatctgttaatgtgatgggtctcccagtgaatgtgaagcttg
ttattgagggaatggaagaagtgggatctgatggactggagaaacttgttgaggataaaaggatactttcttctcaaatgtggactacattgttgtcaca
gacaccccatggttaagcaaagaagccaggaattacttaacggagcaagggggcaactggctaacttttttcctccgaggtaa
3' EST: CF785300
[+]ttttttttttttttntggtgttacatatttaatttaaaaatatctctacagagaaagactcatcggttgctgattactgttagacacagttcagatggct
gttgaagaaagttcttgaaggtaagaagcatacagtttggtaccttcaatgtaattgtacttgcttattttctcattttgaccatgaggagcatcatctg
gtccaccaattcctagtagcattacactctttccaagtacatcttccaaagttttagcaatgggaatagttccaccagctcggatcatgtctgcttccag
gttgaacactctttttactgctcttctagcagccagatattgtggctcattcatgtctgccaaccaaggttttgctccaattaccatttttacctttatt
ttattagggcttttcctttcagaaaatttggcctccaagtaatcagtaacctgtttttctacaacagatggatccatgttaggaacttgacgcatagaga
actttccaattacttttgcagggattacagttttagttcctgttccacagaaagccccctcaatcccatgaattgttagtgaaggatagcgccatctatg
cataagtaaatcttcttttgtatcatgaaggaattgcgtgacccctgtgtcagcttgcatttcctgttgactgaaatcaagatttttgtaccaatctggt
ttcattctcacccacaggtgcccacagccctcataaatccctggggaacaaggattgcgacccctttccatctggcaaccgtgtttcagcaaaatatatc
ca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: