XB-CLONE-4350790
Clone Name: IMAGE:7025990
|
Gene:
samm50
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785302
[+]
taattaatccccccccccccccaacatccactggggcagggactgatgggaatgtgatagggtccttagattgtaagctcactgggacagggactaatgg
gaatgtgatagggtccttagattgtaagctccactggggcagggactgatgggaatgtgataggaatttgagattgtaagcttattggggcagggactga
tgggaatgtgatagggaccttagattgtaagctcactggggcagggactgatgggaatgtgatagggaccttagattgtaagctccactggggcattaat
gtatatattaatgaaggaatgggaatgatgtataatgtgctgtggaaatgtgtcggtgcaatataaataatagataacaataatctggccttcagacaaa
acttgacggggactgatcatgagtaccacaactttgcaatacaaaacaaaagttaacggcaatatgggaacctctgttttggatacagaaatctgcaata
tgaaattacagattgacaataaaactgaagcctctgcaaaaagtttattcctattgcccctaggtggtaccaagtagtgtatgcacaaatccgcccatga
ccagtttcatggtcctaatattcgctctttaaagtctgtaaacctccagtggttttagcttttgtatcaaccacaaaaggatctttagtacaaatctgca
acctgtttttgtggttgcccctcctcaaggaatgggccaacaccacccgtaatatgtcctgccctggatgaaatcagacttggcattattacctgtgaaa
acggtttgctaggaacat
3' EST: CF785303
[+]tcctagagatttctccactatattgggctggattccttctaactggctttatattaacatgataacagtgaaaaaatctagatggcttataatacaacat
ttatgtcagacaaagacattcttcgacctagttggcaagcagggggccagaaaaggccatctccaccccatggacagttagttagttagacagccatgtc
ccctgtattgggctggattgctcctaacgtgcattatataattataattattataattattattatattatttatgtgaaaaaggagaagagatatgttt
attgattatgttagattacagcaagaatgtattactatttaattttccttgcacatcatgttccagtgaggcaattaaatatattctcatgagtgtacca
ttgagtataaagaagctctaaatgaaagtgtttaatactaggaatgtacctaatccaggattcagtttgggattcggctgcacgcacggtcctttttatg
ccttctgtatgttggtttgcgtattcgaattaggattcggattcagttcggtatcccaaaatagtgggtcagtgcatccctatttaatactaatacaact
atactgatcccagaacttgtgtatgtccagtgaccctactgctatttgtagggtgagcgtgatctgcccttctcagggaaattgttcagaaccatagtgg
cactgtgcttgttcttggtctgtaataataataaacatgggctttctccccccgcagggccttctgtctccattccggtgaatgaaattgttgcggtgca
tcagggnagacag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: