XB-CLONE-4350787
Clone Name: IMAGE:7025989
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Gene:
hnrnpdl
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082729 [+]
GTTTTCCACATTTGCTTAGTGTGTGTGTTCGTAAGGGGCTTTTGTATTCGTCAATTAGACCGTAATCATGACCGGATTTGGTGCCACCCCGGACTTCAAC
GAAGGCTCAAAAATCAATGCCAGCAAAAACCAGCAGGATGAAGGCAAAATGTTCATCGGGGGTCTGAGTTGGGATACAAGTAAGAAAGACCTTACAGAGT
ATCTGTCTCGGTTTGGGGAGGTGGTAGATTGCACAATCAAAACTGATCCAGTGACTGGGCGTTCAAGAGGATTTGGCTTTGTTCTTTTCAAAGATGCTGT
GAGCGTTGACAAAGTGCTTGAAACAAAAGAGCATAAACTAGATGGCAAGCTCATTGACCCAAAAAGAGCTAAGGCACTAAAAGGCAAAGAGCCTCCCAAG
AAAGTGTTTGTTGGCGGACTCAGTCCTGAAACAACAGAGGAGCAGATCAAGCAGTATTTTGGTGGATTTGGAGAAATTGAAAATATTGAGCTGCCAATGG
ACACAAAAACAAATGAAAGGAGAGGCTTTTGTTTTGTCACCTATACAGGCGAAGAACCAGTAAAGAAACTTTTGGAAAGCCGGTTCCATCAAATTGGTAC
AGGAAAGTGTGAAATAAAAGTTGCACAGCCCAAAGAAGTCTACAGGCAGCAACAACAGAAGCAGCAGAAGGGAGGAAGAGGAGCAGCCACTGGAAGGGGT
GGTGCCAGAGGAAGGGGCAGAGGTCAAGGTTGGAATCAAGGCTACAGCAACTACTATGATCAGAACTATGGAAGCTATGGAAACAATGGTTCTTATGCTG
ATCAAGGGTATAATAACAGTTATAGTGGTTATGATTACTCTGGATATAACTATGGAAGCTATGGATATAACCAAGGATACACAGACTACAGTGGGCAACA
AAGCACATATGGCAAAGCAAGAGGTGGAGGAAACCATCAAAACAATTACCAGCCTTACTAAAGCGGTGACTATTGAAACCTAAGGTGGCAATGGATAAAG
AGACTTAACATCCTGCGACAATGCCGGGAGCCGCCATCATGTTACTCTTTTGAGAGGACTATGACTGAGGCACTAAGTGTCTTGCTGTAAATAGAAGCTG
GGATTTTTTTTTTTTTTTTATTTCAATGGCATTTTTTTGCCATCTGTATGAAAGAGGTTTTATGTTCAGCAATATTTTTAAGTTCCTTGTTTGTGCTTGT
GAAGTGATCGGTGCATTCAATTTTGATGTAAATGAAGTTTGTAATAAAAGTAAGAACAGTTAAAAAAAAAAAAAAAA
5' EST: CF785305 [+]taattaatcccccccccccccgttttccacatttgcttagtgtgtgtgttcgtaaggggcttttgtattcgtcaattagaccgtaatcatgaccggattt
ggtgccaccccggacttcaacgaaggctcaaaaatcaatgccagcaaaaaccagcaggatgaaggcaaaatgttcatcgggggtctgagttgggatacaa
gtaagaaagaccttacagagtatctgtctcggtttggggaggtggtagattgcacaatcaaaactgatccagtgactgggcgttcaagaggatttggctt
tgttcttttcaaagatgctgtgagcgttgacaaagtgcttgaaacaaaagagcataaactagatggcaagctcattgacccaaaaagagctaaggcacta
aaaggcaaagagcctcccaaaaaagtgtttgttggcggactcagtcctgaaacaacagaagagcagatcaagcagtattttggtggatttggaagaaatt
gaaaatattggagctgcccatggacacaaaaacctatgaaaaggagaagctttttgttttgtccaccctagtacaggcgaaaaaactctgttaaagaaaa
ctttttgggaaaagcccggattccatttcaaatatggttaaaaggaaaaagtgtttgaaattaaaaattttggaccagggcccaaaagaaaattcttaca
gtgggctacctaactaattcttaattcatttcgcaaagtggttagggaaggaagtgatttatgccccactcggaaaaatgggggcgtgttggccccatat
aagcaaagagggcctcgaaaagtgtcctagaggtgttgggaaatctcaaaaggcttaagaggtggtgacttatacttttggaattaagataaaaattata
gcgaaaataccaataggtta
3' EST: CF785304 [+]tttttttttttttttaactgttcttacttttattacaaacttcatttacatcaaaattgaatgcaccgatcacttcacaagcacaaacaaggaacttaaa
aatattgctgaacataaaacctctttcatacagatggcaaaaaaatgccattgaaataaaaaaaaaaaaaaaatcccagcttctatttacagcaagacac
ttagtgcctcagtcatagtcctctcaaaagagtaacatgatggcggctcccggcattgtcgcaggatgttaagtctctttatccattgccaccttaggtt
tcaatagtcaccgctttagtaaggctggtaattgttttgatggtttcctccacctcttgctttgccatatgtgctttgttgcccactgtagtctgtgtat
ccttggttatatccatagcttccatagttatatccagagtaatcataaccactataactgttattatacccttgatcagcataagaaccattgtttccat
agcttccatagttctgatcatagtagttgctgtagccttgattccaaccttgacctctgccccttcctctggcaccaccccttccagtggctgctcctct
tcctcccttctgctgcttctgttgttgctgcctgtaaacttctttgggctgtgcaacttttatttcacactttcctgtaccaatttgatggaaccggctt
tccaaaaagttctttactggttcttcgcctgtataggtgacaaaacaaaagcctctcctttccattgttttttgtgtcccatggncacctcaatattttt
caatttctcccaaatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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