XB-CLONE-4350773
Clone Name: IMAGE:7025984
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Gene:
eef1b2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077005 [+]
GCTTTTCTTTACCCGTGTGTGCTAGGCTTCTTGAGTGAATTGCTGTAATGGGTTTCGGCGATCTCAAATCCCCTGCAGGCCTTAAGGTCCTGAATGAGTT
CCTGGCCGACAAGAGCTACATCGAGGGATATGTCCCTTCCCAGGCTGATGTTGCAGTATTTGATGCCCTCTCTGGTGCGCCCCCTGCTGACCTTTTCCAT
GCTCTGCGTTGGTACAACCACATCAAGTCTTATGAAAAGCAGAAAAGTAGCCTGCCTGGTGTGAAGAAGCCTCTGGGAAACTATGGCCCTGTTAACATAG
AAGATACTACAGGCAGTACAGCAAAAGATACCAAGGAAGAGGATGATGATGATGACATTGACTTGTTTGGTTCAGATGATGAAGAGGAAAATGAAGAATC
AAAGAGGGTCCGCGAAGAACGCTTAGCACAGTATGAAGCAAAGAAGTCTAAAAAACCAGCACTTATTGCCAAATCATCCATTCTTCTTGATGTGAAGCCA
TGGGATGATGAAACGGATATGGCCAAGCTAGAAGAGTGTGTCAGAAGTATACAGATGGAAGGCTTGGTGTGGGGAGCATCAAAGCTTGTTCCTGTTGGAT
ATGGCATTAAAAAGCTGCAGATCCAGTGTGTGGTTGAAGATGACAAAGTTGGCACAGATGTTTTAGAGGAGAACATCACTGCATTTGAAGACTTTGTGCA
ATCCATGGATGTTGCTGCTTTCAACAAGATCTAAACTGATTTCATGAAGCCCCCATACTTTTACAGCACTTCAAATAAAAACATTTGCTTAATGTGAAAA
AAAAAAAAAAAA
5' EST: CF785313 [+]taattaatcccccccccccccgcttttctttacccgtgtgtgctaggcttcttgagtgaattgctgtaatgggtttcggcgatctcaaatcccctgcagg
ccttaaggtcctgaatgagttcctggccgacaagagctacatcgagggatatgtcccttcccaggctgatgttgcagtatttgatgccctctctggtgcg
ccccctgctgaccttttccatgctctgcgttggtacaaccacatcaagtcttatgaaaagcagaaaagtagcctgcctggtgtgaagaagcctctgggan
actatggccctgttaacatagaagatactacaggcagtacagcaaaagataccaaggaagaggatgatgatgatgacattgacttgtttggttcagatga
tgaagaggaaaatgaagaatcaaagagggtccgcgaagaacgcttagcacagtatgaagcaaagaagtctaaaaaaccagcacttattgccaaatcatcc
attctttctgatgtgaaagcatgggatgatgaaacggatatggccaagctagaagagtgtgtcagaagtatacagatggaaggcttggtgtggggagcat
caaagcttgtttcctgttggatatggcattaaaaagctgcagatccattgtgtgggtgaagatgacaaagtttggcacaaatgtttttagaagaagacat
cactgccatttgaagactttgtgccatccctgggatgttggctgcctttcaccagatctaaaactgaatttcatgaagccccccctccttttaacggcac
ct
3' EST: CF785314 [+]tttttttttttttttcacattaagcaaatgtttttatttgaagtgctgtaaaagtatgggggcttcatgaaatcagtttagatcttgttgaaagcagcaa
catccatggattgcacaaagtcttcaaatgcagtgatgttctcctctaaaacatctgtgccaactttgtcatcttcaaccacacactggatctgcagctt
tttaatgccatatccaacaggaacaagctttgatgctccccacaccaagccttccatctgtatacttctgacacactcttctagcttggccatatccgtt
tcatcatcccatggcttcacatcaagaagaatggatgatttggcaataagtgctggttttttagacttctttgcttcatactgtgctaagcgttcttcgc
ggaccctctttgattcttcattttcctcttcatcatctgaaccaaacaagtcaatgtcatcatcatcatcctcttccttggtatcttttgctgtactgcc
tgtagtatcttctatgttaacagggccatagtttcccagaggcttcttcacaccaggcaggctacttttctgcttttcataagacttgatgtggttgtac
caacgcagagcatggaaaaggtcagcagggggcgcaccagagagggcatcaaatactgcaacatcagcctgggaagggacatatccctcgatgtagctct
tgtcggccaggaactcattcaggaccttaaggcctgcaggggatttgagatcgccngaaaccattacagcaattcactcaagaagcctagcacacacggg
taaagaaagcgggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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