XB-CLONE-4350770
Clone Name: IMAGE:7025983
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Gene:
sumo3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785316 [+]
tattaatcccccccccccccgagagaggaaaaaaccttcacagacaggaaaagagcgggagagctagaaaagggcaatcatgtctgaagagaaacccaag
gaaggtgtgaagacagagaatgaccacatcaacctgaaagtggcagggcaggatggatctgtggttcagttcaaaataaaaaggcacacaccactcagca
agttaatgaaagcttactgcgacagacagggcctatcaatgcggcagataaggttcaggtttgatggacaaccaatcaatgaaacagacacacctgcaca
gctggagatggaagatgaagataccattgatgtgttccaacaacagacaggtggtgtgtgctaagcactgaacaagttcaatctccagtatggcaggagc
tcaaattccctccatatgcctcgtttctcacctatgccccttggatttgctgttaaatagttaacacagaacaaacctgctgatcacacgacacttctga
aaacgttggcggactctcccaggggagatattcaatcagaaatgcagttttcttttccagctgaacgtgccagaagttgtaaaggggccaatctaaagca
ttgcctttctctgctggaagttttcacgcttttttgggcaggactgttggtgttacaaaagttctgtttaaattttttcccccgtgtatctgtcgatgtg
aaaaaattgggatcccttggcttggaataatttgtttgaagccaggttccctttgccacgaagacggggcgggatgtttttggtgaaatgtcgtgctgta
gcacgttgggttatacgcgttgaagattggccttgttgacctctactagaatgg
3' EST: CF785315 [+]tttttttttttttttattttttgttcactagagaggcttctaagggtttattgcaattttgtaatgcttagatgtcaacataaaacatctgtaatcataa
atacagtacaactctgctgcaaaagtaaacagaaattctgacttttctttattttgttgaaagaatacatttatccagaaagtacaattaaaaaaaaaaa
aaagggaccacactatagacaactgagcatcataattcaaaatcttagagattgagggagggaattttttttatatgtatctttggctggcactgtaaaa
ctagcacaaagggaaaacctgaagaacattgttggaataaaactactaacttgcagtattgaacatcttttgctactgaacactgctaggaagagcacgc
tcagatatataaactacaacttcaagaaggattcttaaagggaatgtagagattttaaaaccgttttttgggacccataattcgcacactatagtcggga
acaccaaaaatttctgacattgaatggaccaccaccaaagagcccatgctgtcagtttttatttatttattttttttaatctggaactggaaaaagtgct
cctgtgcagcagataaaaagcagggtgagccattgacaaatgtagtaaaacgtctgaatttttgaagtgccaaaattttgcactcacgcatctagcctga
tttgcagaatggtaaaacctctgctgctattagtatattagaaagcatgatttttgtctcgcataagaaaaagtggggtaaaacctgttgggtccaagca
gtcagcagaaatgtgcttcatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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