XB-CLONE-4350764
Clone Name: IMAGE:7025981
|
Gene:
eif3k
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785320 [+]
taattaatcccccccccccccccttttctttttcctctccgctgctggtaacatggcttcgtttgatcaaatgcgagcaaatgtggggaaactgctgaga
ggtatagacaggtacaacccagagaacctggctaccttggaacgctatgttgagacccaggcaaaggaaaatgcctatgatttagaagcaaatctagcag
tgctgaaactctatcagtttaaccctgccttcttccagaccacagtaactgctcagatccttctgaaagccttgaccaacctgccccacacagacttcac
tctctgcaagtgtatgattgatcaagctcatcaagaggaacgtcccattagacagattctgtacttaggtgaccttttagaaacatgtcatttccagtca
ttctggcaagcactggatgagaacttagatcttatagatggtattacaggctttgaggattcagtaaggaaatttatctgccatgttgtgggaatcactt
accagcacattgaccggtggcttttggcagagatgctgggagatttgtcagagccccagctcccagtgtggatgagtaaatatggatggatggaaaagtg
aaaaggggaaaattttttggttgcaccccccgaagaaaaaaaatccnaaccttaagaaacataatttgaaaaaaaaaaaaacttttttaaaagggtttct
ctgggagaaaaaagggccaccttctccccaaaaaagattttctttttaaaaacaaacgcccaaatgtggtgtttttttttttttcccacaaaaaaagagg
ggtgtgttccccccagcgggcgctaataaaaaaaatacccgcggggagacctcatcttttt
3' EST: CF785319 [+]tgggaaaagtagtccagaagtctgtgtttcttataaaaactcctcagaattgttaatgcatgtatattacagagtgttatttttgagaggggaaactata
attacattagtttccctttaaaaggaagactggctgcagctgaataacccacctctaagttgtcacattcttgtggtcagtgaaccaagaggttcaataa
atgcagttccattgtgatgtactatagctttgccactggctgaagagacaaacatcctagtcttttcagtcttctttatcatagacctctttactccata
ttgccatttgcatagacagaattaattgattcactgattttattagccagctgcaacactcattctgtacataaaataacatttagcttgttataagaaa
acttattgagaagatgccattattccagaaacactgtcaaagtctatcttctcaactatgttcttaggtttgatattttcttcctggttgcatacaaaaa
ttttgccattttcactttccatccatccatatttactcatccacactcggagctggggctctgacaaatctcccagcatctctgccaaaagccaccggtc
aatgtgctggtaagtgattcccacaacatggcagataaatttccttactgaatcctcaaagcctgtaataccatctataagatctaagttctcatccagt
gcttgccagaatgactggaaatgacatgttttctaaaaggtcacctaagtacagaatctgtctaatgggacgtttcctcttgatgagcttgatcaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: