XB-CLONE-4350759
Clone Name: IMAGE:7025979
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785323
[+]
taattaatcccccccccccccgctcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaaggaaaag
actcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaaccggacatcttatctacaaatgtggtggtatcgacaagagaacca
tcgaaaagtttgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggtatcac
cattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatcactggt
acctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctcc
ttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagccagaaaagatatgaggaaatcgttaa
ggaagtcagcacatacatcaagaagattggttacaaccctgacactgttgcctttgtacctatttctggatgggaaggtgacaacatggctggagcccag
cgccaatatgccttgggtttaaggggggggaaaatcctcaccgtaaaagaggggatcttgggcaaccgggaactaaccctgggctgggaaaagcttcttt
gaaacgtgca
3' EST: CF785322
[+]tttttttttttttttcttttaacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaacac
taataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgactat
taaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctttc
tgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacgga
cagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcagcatcaccagacttcaggaa
cttgggattgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaaatgtgagctgtgtggcaatccaacac
agggggcatatccagcaccaatctgggcctggggtggttcaggatgaataacctgttgctggtaaaggtaccagctttccattgggtggggtcaattcct
gctgtccaccagcaacctttgcccaccaaacggaaggtcctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: