XB-CLONE-4350753
Clone Name: IMAGE:7025977
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Gene:
ocm4.5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785327 [+]
taattaatcccccccccccccagaagtgttaatactcaaaaggttgctcagagccctttgctcctgttaaagaggaattcagatttaccatggcattcgg
tggaatcctgagtgaggctgacatctctgctgccctgcagaactgccaagcccctgactccttcaacttcaaaactttctttgcccagtctggcctgagc
agcaggtctgcagatgatgtgaagaatgtctttggcatcctggaccaggacaggagtggcttcattgaggaagatgaactaaaactgttcctccagaact
tcagcgcaggtgccagggctttaactgatgctgagaccaaggccttcctggcagctggagactctgatggtgatggcaaaattggagttgaagaattcca
ggctctagtcagaccttgaaaatgaagaccacaaactttcatgaaagatgaagttgcaggaattttggcttttcttagttaaattgaaagaagtggaacc
tgggttgggcagaatgcagaaaacctaatggacaagcaaaggaagggcttttgtgctgcataaaaagaaacgttgtcggggaaaaccagcaaattttatc
taagaaattggccctaatttaacaggtaattttatttaaacaatggttattatacccaactggggccttctggattcataatcagcaatggaattttttg
gtaaaatttaagcattgtggaattccttccttttacaagaccggaggggcaggccaccctttaaaaaaaccttcaaaagactggtccaagtaaaaaaaac
aattttttttggtgtaaagtaccaaatagattcacataatgtggaaagggggacttaatgaaaagcgactgcacacaacgcacttatacaagaattatat
acacagagggggtttctc
3' EST: CF785326 [+]tttttttttttttttgagagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcagg
ggaaatttctgttcatttgttcctttatctttcccgactgcccttgcatttatgacactgttgaagaaacgaccttcccatctaccacctcttcgacaat
ggtttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttcc
ccatccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccctgtccgacc
tgatctgcaaaagctgttcctctaggcctgctgaatattgtttctgaatctgcagaaattgcgccttaatagaagccctttaaatccattgagggttgcc
tttccactgaattgttttcctttggccagcctggggatttgcaacctcaattttccaagcttcttgaaatggaccgtttttcaccttcaaggattttcac
tccttggcctgggccttgcacttgttttcaacccccaactgaaaattctctttttttaaactcacccgcttccttcttggggggaacccccaaattctga
aatcccttaaggggttctttcttcagccacggtattttttataaggtcccgactcctcaaggtttaattttaaaaaagtttgggtttacaataaccccct
cctgggtgaccccggggttccacatacccaaacatcttctccttaaggccgcgggcaccactgggcgcctttgtggaaatgcaatactaccctccctctc
ccactaaggggattccttcttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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