XB-CLONE-4350750
Clone Name: IMAGE:7025975
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785329 [+]
taattaatcccccccccccctatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagagaaa
ggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacagagac
ggcatcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcatctg
gccccatcaacttcaccgtcttcctgaccatgttcggcgagaagctcaagggtgctgatcccgaagacgttatcactggggcattcaaggtgcttgatcc
tgaggccaaaggatccatcaagaagcagttccttgaggaactccttaccacacagtgtgacagattcaccccagaggagatcaaaaacatgtggacagcg
ttcccccctgatgttgctggcaacgtagactacaagaacatctgctatgtcatcacccacggagaggaccaagaccaggaaataacaggcccagtcttgc
cctcctgatggaggggggtgcgccatcaagccccaacaccaaccttggcggaaaattggcgtcctatttttaaaaacctcattcccattcggaaaacacc
aacgggcaattttaatttccttccaaaggggggtggtctctaaaaagggggaacccccccctccaggttagatacctgtggccccactttttcccggcct
ggtccccctttttcccctacccccccgtttatacacccctactccctttaaaaaattttttccct
3' EST: CF785328 [+]tttttttttttttttgtataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagggaggg
taaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttgtgtct
ctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactgggcctgt
ttattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccacatgttc
ttgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcagggatcagcacccttg
aatgccccaatgaaaaagtcttcgggatcagcacccttgagcttctcgccgaacatggacaggaaaacggtgaaaattgtagggggccaaaatgcctctt
tgaaccatctcttccaaattctttattcttcacattcaggggggcccaaaaatgccaaatggggccccccaggtctttccttgatcaaagaagccggtcc
attggtctgaagaaaacactggggagaagcctccatgaaactccatggaaacgggggtttgggccacaaattgggacaacacattttgaaaaaacacccc
ttccgaggggcccgttaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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