XB-CLONE-4350745
Clone Name: IMAGE:7025973
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785332 [+]
taattaatccccccccccccccccccgcacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatg
aaggaaaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggt
gaaggatcgggaaagcaagagatccagaggctttggctttgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtct
gtagatggccgtcaaatccgtgttgatcaggctggcaagtcctccaatgataggagaggtggttacagaggtggctcttctggaggcagagggttcttcc
gtggaggcataagccgatgtggtggtggaaacagaggatatggtggaatctcccgtctttgaaaaaccgaagttggaggttaccaaacccactggaatcc
acttagtttttagaaggggacccacaacgccggggggaagctttgggcacgtgggcccaatctttgttttttttggctaaccttataaaattgggtttac
caacaatttatagcccaatttagggcgtccctcatagatgtttcccgccgaatatataaagcgcatttgtttttttttcaacgcggcaaatctcactaat
attagtggagtttattttttcccaaaaacatttcaattacaaaatgatatattcctttaatacagagaaaccttgagtacttcatgagaatatcaaatgt
ttcccggccgttgtcattcgatccatgtggggcacatcgcaataaataactcctcctgtgacagattattgtcactctttggtgtttatctatatctccc
tttatttgagagggtcgtgcgtaaaaaatatcaccgctcctctgcgggggaatcttgtgtccttctattatcttagcgggtgtgtagtatataatctcgc
catagagcgcaccccccaatacgtan
3' EST: CF785331 [+]ctgctttggtaacctccacttctgttttcaaagcggctgcttccaccatatcctctgtctccaccaccacctcggcctctgcctccacggaagaaccctc
tgcctccagaagagccacctctgtaaccacctctcctatcattggaggacttgccagcctgatcaacacggatttgacggccatctacagacttcccatt
cattgccatcatagcatccttggcatcttcaggattctcaaatgtgacaaagccaaagcctctggatctcttgctttcccgatccttcaccacaaccact
tcagcaacctgtccatatttgctaaacacttgctccaagctttcctctgtggtttcaaaattcagaccaccgacaaagagttttccttcatcacaagaca
tggtgagacgttctatgatcttcggacgatacaacacaagttggaagccgaatgcgaagtgcgggggggggggggggggggatttatttgttttccctgc
cagacaaacaagcgccttgttttcggtgccccgaaatataagcgaagggtcatgtagatccggagagggagacccccctgggcttaacaagggggacggg
ggagggatgcgtaataattatctccctacactggtagtgagatgtgaaggtatttggttaatgtaataagagaatacctacgttagtgtgcgcgacgttc
tgaggtgagggggactcttagaccgcaactcggatgcggcgattcatatgacgttttcagccgagggcgaatagaggaatcgcgtcgtttta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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