XB-CLONE-4350740
Clone Name: IMAGE:7025971
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Gene:
pbx2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785335 [+]
taattaatcccccccccccccactcctcttttcccagcttcaatacagcgctactggctttttttagggatcccagaaaaaagcgaagcaggattgcacc
ccaccccaaaaatcatccggactgagctgtccgagtcttagcgatttagccgcccaggcgcgtcggacagtgcaacacggagcgatctatcggaggagcc
acttgaggagaaaggaagcctgggcgcgatctgtatacactgcctacagtcggggcttcgtaggcgctgctgggagaggaggcgaattccttccgagcaa
atcgcatggtggaagcacagagcgatcagtagagcaggacgggactactcggggcaggaggagagttctatgaggggcagggggagttgtacaaggagac
agagctgaggggacaagtgagaaacacacataaaaacaggagagcgatccctcgctggtgctcagatggatgaacaaggcaggctgatgcaggccagggg
ggtggggattccaggacaccccatccatggggggcctcanactctcactcctcatcctatgcatgaacccccggcagacnatggggagcccaggaaacag
gatatcggtgacatattgcaacagatcatgactatcacgggacagagccttgatgaagcccaggcaaaagaacatgcttttgaactgccacaggatgaaa
cctgcacttttcaatggcctttgtggaaattaaagagaaaacagggtttgagtatttcgtaacaccccaaagaaggaaaaagcctgtttgacccttcaga
ctcatggcgccttgggaccaaaatgtttacc
3' EST: CF785334 [+]ccacctttctctggtcctgctacaccttctgctagtaacatattgtccaagcgcatgagctgagggtcaacaggctcttcctcttgggtgttacgaatac
tcaaccctgttttctctttaatttcacaaaggacactgaaaagtgcaggtttcatcctgtggcagttcaaagcatgtttctttgcctgggcttcatcaag
gctctggtccgtgatagtcatgatctgttgcaatatgtcaccgatatcctgtttcctgggctccccattgtctgccgggggttcatgcataggatgagga
gtgagagtttgaggccccccatggatggggtgtcctggaatccccacccccctggcctgcatcagcctgccttgttcatccatctgagcaccagcgaggg
atcgctctcctgtttttatgtgtgtttctcacttgtcccctcagctctgtctccttgtacaactccccctgcccctcatagaactctcctcctgccccga
gtagtcccgtcctgctctactgatcgctctgtgcttccaccatgcgatttgctcggaaagaaatcgcctcctctcccagcagcgcctacgaagccccgac
tgtaggcagtgtatacagatcgcgcccagccttcctttctcctcaagtggctcccccaataaatcgctccgtgttgccatggtccaacacgcctgggcgg
ctaaaaccctaaaaactcggaaagctcatccccggagaatttttgggggggggagcaaacccggcttcccttttttttctgggaacccctaaaaaaaagc
cagc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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