XB-CLONE-4350735
Clone Name: IMAGE:7025969
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Gene:
sh3bgrl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077071
[+]
GGATTCTAGTGGAAGCTGCTAAAGCTGCTGGAAGGGGAGTTCCCCAGTGTGGGATCCAGTTTACATACCAGACAGACGGCACATAATAAGCAGGATAAGC
CGCACCATCCCAGGGAGCAGCTGTCACACCTGCTGTAAGAAGATCTCCTTGCCCGGCTGGCACACAGGGAGAGGATGGTCATAAAGGTGTTTTTAGCCTC
TTCCTCCAGCTCTGTTACTATCAAGAAGAGGCAGCAGGAAGTTTTGCAGTTTTTGGAAGCCAACAGAATTGAATATGAAGAAGTGGATATTACTATGTTA
GAAGAAAAGAGACAGTGGATGTACAAAAATATTCCCAAGGACAGGCTTCCTGAGCAAGGTAATCCACTGCCTCCACAGATCTTCAATGACAATATCTACT
GTGGGGATTACGAAAGCTTTTTTGAATCAAAGGAAAGCAACACTGTCTTCTTGTTCTTACAACTGAAATCAAGGCCAGCACAAAAGGAACTTTAGAAGAA
TTTAATATTGGAAAATAATAAGTACAGGACACTCAGCACAGCTTTGTCATCTGTTGTTCCGAGGAGTCGATTGTCAAATTTCTTTGCCAAAAATGGCACC
CTGTGACAGATCATGGACTGCATCACCAAATGATATATTTTGCTATAATGTTAGGTGCAGCAGATGTCATCGAGAATGACTCAAGATTAACACTGTAAAT
GAGTTTCAGTCGAGCTTGGCTTCTGGAGAGGTTTTCTTCAAACACCATCACCTTCATATCAAGTATTCACCCTACTCCTTAGATTTGAAATTGACTTTAT
ACAGTTGTACTTGATGCTTTAATTCCAGCTAAATGTCTTTTTATTTGGTTTGCCTATTGCACTGCTACTTTTAGCATCTGGACTTTGGTTTCTGTGTTAT
GTGTATGTATGTATATATATATAATAACAGTTAAGCACCACAATAAAAAACTGTTTTGACCAAAAAAAAAAAAAAAA
5' EST: CF785338
[+]taattaatccccccccccccggattctagtggaagctgctaaagctgctggaaggggagttccccagtgtgggatccagtttacataccagacagacggc
acataataagcaggataagccgcaccatcccagggagcagctgtcacacctgctgtaagaagatctccttgcccggctggcacacagggagaggatggtc
ataaaggtgtttttagcctcttcctccagctctgttactatcaagaagaggcagcaggaagttttgcagtttttggaagccaacagaattgaatatgaag
aagtggatattactatgttagaagaaaagagacagtggatgtacaaaaatattcccaaggacaggcttcctgagcaaggtaatccactgcctccacagat
cttcaatgacaatatctactgtggggattacgaaagcttttttgaatcaaaggaaagcaacactgtcttcttgttcttacaactgaaatcaaggccagca
caaaaggaactttagaagaatttaatattggaaaataataagtacaggacactcagcacagctttgtcatctgttgttccgaggagtcgattgtcaaatt
tctttgccaaaaatggcaccctgtgacagatcatggactgcatcacccaatgatatattttgctataatgttaggtgcagcagatgtcttcgagaaatga
ctcaagatttaacactgtaaaatgaattttcaatcggaactttggcttcctgggagaaggtttttctttcaaacaacccttcaccctttctctattccag
gttatttccaccccctacctccccttaagaaa
3' EST: CF785337
[+]tttttttttttttttggtcaaaacagttttttattgtggtgcttaactgttattatatatatatacatacatacacataacacagaaaccaaaacataag
atgctaaaagtagcagtgcaataggcaaaccaaataaaaagacatttagctggaattaaagcatcaagtacaactgtataaagtcaatttcaaatctaag
gagtagggtgaatacttgatatgaaggtgatggtgtttgaagaaaacctctccagaagccaagctcgactgaaactcatttacagtgttaatcttgagtc
attctcgatgacatctgctgcacctaacattatagcaaaatatatcatttggtgatgcagtccatgatctgtcacagggtgccatttttggcaaagaaat
ttgacaatcgactcctcggaacaacagatgacaaagctgtgctgagtgtcctgtacttattattttccaatattaaattcttctaaagttccttttgtgc
tggccttgatttcagttgtaagaacaaaaagacagtgttgctttcctttgaattcaaaaagctttcctaaaccccacagtagatattgtcattgaagatc
tggtggaggcagtggaataccttgctcaagaagcctgtccttggggaaaatttttgtacctccaatggcctcttttcttccaacataataatattcccct
tcttcatatttcaattctggtgggctttccaaaaactgggaaaaactttcctggctgcctccttcttggataataacaaaaacttgggagggaaaaaggc
caaaaaaaccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: