XB-CLONE-4350732
Clone Name: IMAGE:7025968
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Gene:
ston1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785339
[+]
taattaatcccccccccccccgctcattgcaagctttacaagctcggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactgagagc
aacaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaatgac
caaggaaaccggaccacacctagttatgtggcttttacagacactgagagattgattggagatgcagctaaaaatcaagtcgccatgaaccctataaact
cacattttgatgcctagagactcattggccgcagatttcaacaagcagtccgttcagtccgacattagaaaccctgggccatccccctgaatatgagggg
acagttggaaaaactgaagggcccaggcgagagatttaaagtcaggagagccacgtttttttgggcgccgggggaaaaattgcacgcccgtggggtttct
atgttgaattagttaaataacaatttgacctgtatcccacaaatcattgggctctaataccctttttatccacccccctattgggtcaaataattaacag
ttttgctacggaaggggtcgttttttcgcggttgattattatttttggaggcgcacccacggcgcctcacacacacttacttaagatagtgccccgggtg
gtttattccaatataagatataattgaagggggcgttccaaataaggataaaagtgtggtatattaaaacgacctcggacgagaagaaaaagttcatcag
cgccccactattaaccacgtgctcaataatatatatccccaataagtgtgaacactgacgttaggatagaatgtatggaggggtggaggaccaccactaa
aatcgcaatagaccgttgcggtctccgccctatatgtgcgagagaagacttattctgttgtgttatgttacctccgaccagccgccgngatatgtgggtg
ctttgtggtccatacgtgagtgtgacataggataaattagccgcgttattacgtatagaggtatgaagacaaggtgtcttctcgttatgaacggcgcgca
caccacccgtagatt
3' EST: CF785340
[+]tttttttttttttttggcaattcaattcattttattggtctggacccaatttgtttgtacagtacagatactatggttttcctgacttttggagttaatt
caagtattaatgccccaaaattgacatacagcagcatacatgagaaaatttgaacagctagtacgaaatttgtgtcctaggagttacaagacaaaaaagt
ggaatgcagcttttatttagtcaacctcttcaatagttggaccagaggatgctcctcctgatggggcaccaccagcacctgggaagccaccaggcatacc
acctggcatgcccccagcactttgatatagctttgtaataatagggttgcacaccttctccagctctttttgttgatgttcaaactcttctttttctgca
gtctgatttttgtccagccaagatataacttcattgcatttatccaggattttctgtttgtcttcatcactaattttttctttaagtttctcatcttcaa
cagtagccttcatgttaaatgcgtaagactccagggnaattcttttgaagtacccttatccccttttgctctcatcttcagctttggacttctcagcttt
cttgaaacatacgcctcaaaatcctcccttgctggaaacgggcttttgtcattggggaaattggggattttgttccccccttcccgggggtccttgccca
ccggccaaaaaccattttaaaaaaccctttgggcatcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: