XB-CLONE-4350729
Clone Name: IMAGE:7025967
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Gene:
tpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785342 [+]
taattaatccccccccccccttttgaagtggaagggaagatcatccaaagagtagaaggtgcaattgatgatgcgcttattggtggaaatgcttcagctg
agtgccaggaggaggacatcggtggtgcaacaacagtcagcggggtggacatagtgattaatcacaaacttcaagaaactggtttcaccaaggactctta
caagaattacattaaggattatgtaaaactagtcaaggccaaacttgaggagactgatcccgacagagtaaaacccttcatgaaaggaattcaagataga
gtaaaactcatacttggaaacttcaaaaactatcagttttacactggagaaagaatgaacccagatggtatggtggctctgctagattaccgtgaggatg
gtgtaacaccttttatgatcttctttaaggatggcttgatcagtgagaaatgttaacattcaatttggttcttccatcttggctgctgcttttcatccat
gaacaccaggtctaagcaagatcttactgactcctaaaggactgatgatacattgagcttgtttcttcatggccctaaattattcaagtggaggcttttt
ttgtttttttaattgaaatcctttttgtcttttagagtaaataaaagcccataattctccactgtaaacattgcccccttgggttggatctgcaatttac
ttttggaaaacttgggtccttgtgcgatttaaagattttgtttgcccccaaaagcttntgtacttttatccaaactgaactggccaaaaaccttttttgt
cttaataaaagccggaaacctttccccatctttgtgtgggaaaaaattccttgttcaactacacttt
3' EST: CF785341 [+]tttttttttttttttacattgtttaatcttgtttttatttaggatcagtaagaaaaaaagggaaagatcttctgtgatggtagagcctgttaacaggcaa
cagtcaatgttccaaatccaaacatgttcttgtgcaaacaacctgcagtgtaaccaaccccttcacagccagaggtatttgcaacactgttgaaaggcct
tctggcagtggaggagtctaaaaacctgaagccgagaggcagattgaatactttggctgtttccttaagtgtgcagattgtccacaaactgaaggtccgc
ttcttacaaaatgttctggcagtcagtctgataagtcaaagcttttgcgcaacaaatcttaaatgcacaagacccagtcttcaaaagtaattgcagatca
aaccaagaggcaatgtctacagtggagaattatgggcttttatttactctaaatgacaaaaggatttcaattaaaaaaacaaaaaagcctccacttgaat
aatttagggccatgaagaaacaagctcaatgtatcatcagtcctttaggagtcagtaagatcttgcttagacctggtgttcatggatgaaaagcagcagc
caagatggaagaaccaaattgaatgttaacatttctcactgatcaagccatccttaaagaagatcataaaaggtgttacaccatccctcacgggtaatct
agcaaaaccaccataccactctggggttcatttctttctcccagggtaaaaacgaaaaagttttttgaaatttcccaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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