XB-CLONE-4350720
Clone Name: IMAGE:7025964
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Gene:
XB5767169
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785347
[+]
taattaatccccccccccgaggtactgcagggggagaagaaagaagccggacattaacccgacccactgacaggatgaagtgctttgtggtcctgctggt
ttgcatctccattgggtgggtccattcctgcaaattcaggcaggaagaaagtaatctaataaagggagcaactgctaaacaaagctccacttctagaccc
gattacttggttgacagagcctttgatggtatcagtgatcactacatgatgagacacccttgtgcggaaactaggagagaacattccccttggtgagagt
tgcaccttaaacataatttaccagggtgatttcattgctccccgtcagcccgatggattgcagccttcataaactggttggggagctcctaatccgcccc
gccacatcaccccggataaaaaatcaaccgttgtgctgctggcgcttagaaaccgcacggttgtttctgtatacccttcaacccttttggccgccccctc
tcgtttggtatagtgttgacccatttgttgcatttctctggacccccccccccttatggtcccgtcgccggccaaaataatcttaccgcgccccaaatcg
gtggttacacacgggagagacgatttggtgccgcctctgtcatagggttggttcttctgtttgtatgacaaaaggtgctataatgctcccgcgcttgttt
ttttttttattttgcgctcagccaaactggatcacgtcgcacaccaatacatctagctatttattttagatatatcagtagcgggatcctatagatacat
tacttcatctgggcgcaatanagaatagcgcgcgataactgttttcatttacacaggcgctttcgacgcccgcgcatctaggaagtgaggattcactcgc
ccgtccagtgtggggttaggtttagatacgtacttcgttatgtcgccggcgggcgctcgntntaaaaaattaacgtaagacattccgcgcgcctgtgctg
atattatgatttagcggccgtgatgtgaccgctcttttattttgtgatcgttctctctgttgtcgtgtatatgaacactccttttgcggccgattttttt
gttttgtctcacatgtcttaattgccgcgcttttttttttgccgcgcgcgcgtgtgtagttttctcacgcaatgctctagtcgctccgtcccacgattta
attcacataagatatcttaccgagatattagtatatagtatacttcctaccgcccgcgcctctctctn
3' EST: CF785348
[+]tttttttttttttttgaaccagcatttgtatttattgtttcacagcagattcattcaggttaatgagcaaagtcttcttcagcctctaattgaataaact
gcctacgtcttccactactgatgcgtcggaattttcatggtttggtacaacaggtttctattcttgctcatagacctccacttcgcagagctggagatat
tcttcacgcccaggaataaccacagagacatactgaccctccttcccatgacaggacacagtgatttcttcggagtcgtcggttacggtgccgcatactg
ggttgttattatcggctgaatctccaacgcggacctcggctcccaacaggcgctcgctgcagcagtctcctctgttcactatcaccactttccccacctt
gtacctcttcttcaggttcagctgccaccaagccgggttgtcattaccggtgagggaacatgagcccgacatcatattagagtcagtattaccatcgata
gcggcggcagcgttgtacatggggccataagttgagctctgtgtggcttctcccgatcttgcaatattaactctattatgtgctggttcatcttgggata
actttctattggcaccttcactataaaccttcacttcacagattgtgagctgttccacccgcccggggataaccacactcacatagtgaccctccatccc
attacagcacagattgatgggtctgaagacgtcggtgatggtgccgcacactgggttgttattatctgggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: