XB-CLONE-4350715
Clone Name: IMAGE:7025962
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Gene:
XB5767169
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785350
[+]
taattaatcccccccccccccaggtactgcagggagagaagaaagaagctggacattaacccaacccactgacaggatgaagtgctttgtggtcctgctg
gtttgcatctccattgggtgggtccattcctgcaaattcaggcaggaagaaagtaatctaataaagggagctactgccaaacaaagctccacttctagac
ctgattactcagttgaaccagccattgatggtatcagtgatcacaatatgatgagacacccttgtgcagaaactaaagagagaaaattccccttgatgga
attcggccctgaaacacaattacccgttgattaaatgcccctcacacacgccgtaatattaacaagtccaaacgctattttggagcataattcccccccc
cccacactaccttatacacaataacaaataattactcatacaaatataaaccccaacattttattatatctcatctcccctattcacccccccctatttt
tttaaagcaatccctttaatcgcttatacacaaccctcccccattataaacacggtcgccaaaatcactatacacccccaatagagtaaatacaaagaag
aagatttttattaagcaccgagattgcgtatagtccatatttcacacacgactactaaatagcttgctatatttttatatttcttatttacgaacggaaa
gacggcgaagtatatataagatgtatattaacgtccgtacctaaatacagcacacgcaccatngtaattaataggttaatgatattttccgcctccgcgc
ccgactcacatataaaatgaggacacactgtgtttgtaaataatattatattgcacacgttctgtccggcatataagtagaattaagcccgcaacttaaa
atatatacgatctattacccgcccttatattataatcgtacgagtcacggaagaatctactctactcgccctccatattttatatacacaccaaacatat
atggctctgtttataactcgaggtgacgtgattttatatcctacccattgatatgactcacgcgcgccccgctgcataaatatataaactgacacggcga
tggtatatgttactatttgtattgtttcccccttcctcgccttn
3' EST: CF785351
[+]tttttttttttttttatgaaccaacatttgtatttattgtttcacagcagattcattcaggttaatgagcaaagtcttcttcagcctctaattgaataaa
ctgcctacgtcttccacaactgatgcgtcggaattttcacggtttggtacaacaggtttctattcttgctcatagacctccacttcgcagagctggagat
attcttcccgcccgggaataaccacagagacatactgaccctccttcccatcacaggatacagtgatttcttcggagtcgtcggttacggcgccgcatac
tgggttgttattatcggctgaatctccaacgcggacctcggctcccaacaggcgctcgctgcagcagtctcctctgttcactatcaccactttccccacc
ttgtacctcttcttcaggttcagctgccaccaagccgggttgtcattaccggtgagggaacatgagcccgccatcatattagagttggtattaccatcga
tagcggcggcagcgttgtacatggggccataagttgagctctgtgtggcttctcccgatcttgcaatattaactctattatgtgctggttcatcttggga
taactttctattggcaccttcaccataaaccttcacttcacagattgtgagctgttccacccgcccggggataaccacactcacatagcgaccctccatc
ccattacagcatagattgatgggtctgaagacgtcggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: