XB-CLONE-4350700
Clone Name: IMAGE:7025957
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Gene:
slc25a5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785361
[+]
taatccccccgccccagccctaggaagcttttggtgctgcttcttgagcctagcatctgctaacgtaacaccggcatcaccatgactgacgcagccatct
cttttgccaaggacttcttggccggcggtgtggccgcagctatctccaagaccgctgtagcacctattgaaagagtcaagcttctactgcaagtccaaca
tgcaagctttttgatcaccgcagacaagcactacaagggcatcatggactgtgttgtcagaatccccaaagaacagggtttcatgtccttttggcgtggt
aaccttgccaacgtgatccgttatttcccaacccaggccctcaacttcgccttcaaagacaagtacaagaagatcttcctggacaatgtagacaagaaga
cccagttctggcgctactttgctggcaaccttgcttctggtggtgctgctggggcaacctccctctgctttgtctacccccttgactttgcccgtacccg
tctagcagctgatgtgggcaaaggcaatgctgagagagagttcaagggccttggtgattgcttagttaagatctccagatctgatgggatcaagggcttg
taccagggattcaacgtatctgttcagggcatcatcatctacagagctgcttattttggcatctatgatacagctaaaggtatgcttccagatcccaaga
acacccacattattctcaactggatgattgccccagaatgtaaaaagcagtagcttggattttgtctccctatccaatttgaacaaccagtaccgttcga
ccgtaaagc
3' EST: CF785360
[+]tttttttttttttttgatccgtattttttatttcctcaaacctaagttacttaaaaacatgaatgactgaaataaaaaataaaggagcgttgagtcccac
tgaatcagcctgtcactgccaaagggccaaaaactgccaaggaggcacagttgcaggcactaaaagcagtaagagacttaaagggaatgatttattaatg
tggaaagctggtgagatgtaatagaactgatcccaaaataccagccagtaaagattaccagctatattttataaataagggtgtgtttgacaaaaaaaat
aaagaaggtccatgaatgttcaaggtatgttacataatacagcatgccaggtcacagacatctcaacgaggacaagtttagatgtatttcttcagctcat
catacaacaccagcacaaaagcgccacccattcctctgagaacattggaccaggcacccttgtaaaaagccctgctggcctcattccgtgcaatcttcct
ccagcagtcaattgttccaactgtacatgaaggtcagctccctttcctttccaaactggcatcatccttaccaacgacgttactggtgtcaaattggatt
agggaaaacaaatcccagccaactggcttgtttaaaagtctgaaacaaatccattccagccgtaatgaaaaaagggggggggggtttccttgggggaatt
tgggaaaaccataccccttttagcctgggaatccaaataaaaggcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: