XB-CLONE-4350695
Clone Name: IMAGE:7025955
|
Gene:
rps2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785364
[+]
taattaatcccccccccccccttctgatctatcgaaatcaccaaatggcggacgacgccggtggtaacaggggaggtttccgcgggggcttcggcagtgg
tggccgaggccgcggtcgtggaagaggcaggggacgcggcagaggacgtggggcccgtggcgggaaagcagacgacaaggaatgggtgccagtcaccaag
cttggacgtctggtcaaggacatgaagatcaagtccttggaggaaatctatctcttctccctgcccatcaaggagtctgaaatcattgacttcttcctgg
gctcctccctgaaggacgaggtgctgaagatcatgcctgtgcagaaacagacccgtgccgggcagcgaactaggttcaaggcttttgtggctatcggtga
ctacaacggtcacgttggtctcggtgtgaaatgctccaaagaagtggccaccgccatccgtggagccatcatcctggccaaactgtccatcgttcctgtt
cgtagaggttactggggtaacaagatcggcaagccccacactgtgcccctgcaaggtgactggccgctgtggttctgtgctggtgcgtctgatccctgcc
ccccgaggtaccgggtattgtgttctgctcccggtccccaaaaaagcctgctgaagaatgggctggtatttgatgaattgcttacacatttaggccaggg
gggctggcactgcccacctttgggggaaactttcgcctaaagcccaacccttttgattgccccttcctcaaaaaggactttactaggctttttttctggg
aacatctcttggtgcttcttccttgggaaaagggaaaaacctgggttgtttttctatccttataaaatatcctctcccccca
3' EST: CF785363
[+]ttttttttttttttagttggaacaaggttttacttttatttttctttaacagaaaaaaaattaggaggtggcaggaacagcagcagcctgggtcctctgc
acggacacccgggtgtgagtcttggccagatggtcagtgtactcctggtatggagatttagtgaacacagtctccttccagaggtcaggagtcagatagc
tgtaagtctttgagatggcatcaaaggtggctttagcgaagttacccaaggtggcagtgcagcccctggctgatgtgtagcagtcatcaataccagccat
catcagcagcttcttggggacaggagcagacacaataccggtacctctgggggcagggatcagacgcaccagcacagaaccacagcggccagtcaccttg
cagggcacagtgtggggcttgccgatcttgttaccccagtaacctctacgaacaggaacgatggacagtttggccaggatgatggctccacggatggcgg
tggccacttctttggagcatttcacaccgagaccaacgtgaccgttgtagtcaccgatagcccaaaaagccttgaacctagttcgctgcccggcacgggt
ctgtttctgcacaggcatgatcttcagcaacctctcctttcgggaaggagcccaggaaaaaattccatggatttccaaactccttggatggggcagggaa
aaaaaaaaagatttccctcccagggacttgaattcttcaggtcctttgaccagaacgtctcaaacttgggtggaaggggcccccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: