XB-CLONE-4350690
Clone Name: IMAGE:7025953
|
Gene:
eif1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC077051
[+]
CCGCTACGTGTCTCCCCAGCTGTTGTTGTTGAATCTGCTATTGTGCTGAACCAATTCTACTTCACATCTTTCCCCACCGAGGAAACGCAAGCTTATCGTA
TGTCAGCTATCCAGAACCTCCAACCTTTCGACCCATTTGCTAATGCTAGTACCGGCGGCATCTGGCTCCCAGCTGGGTCAGAGGATTCCATCCATATAAG
AATACAGCAAAGGAACGGCAGGAAAACCCTCACCACCGTACAGGGCATAGCCGATGCCTATGATAAGAAGAAACTAGTGAAGGCCTTTAAAAAGAAATTT
GCCTGCAATGGTACCGTGGTTGAACACCCAGAATACGGAGAGGTGATCCAGCTGCAGGGAGACCAGCGCAAAAATGCCTGTCAGTTTCTAACTGAGGTTG
GGCTGGCAAAGGAAGACCAGTTGAAGGTCCATGGATTTTAACGTCTGAGTATTTGGAATTGAACACACGGCTCCCTGCTGCAGTGTGTAGTTGCCTCCGG
CTCCCTTATCACAAGCTTATTCTCCAGTCACTCCTTTATATGAGAAATAACTGTTTTATGGACAAATTGTGGGCGCACAGTGTGTGGTTATAAGCCTGAG
AATGTCTGTACAGCTGCCTGCCCCTACAGGGGCAATGCTTTCCCATTGCTGCCACAGACCCACCGTAACTAAGTATGACTTCTATACACACCAGACTGGG
GAGGTAAGATGAGGCTAAATCTTGCTTTCCTGGTTATGGCTTACAGTAAAGATGGCTAATATTGTGCTGGCCAAAGCAGCAGGAGGTGGCCCATTGAGAC
TTCAGTCCCCAGTAATGTCCAGTATGAAGTGGCTGTTCAGTATTAAACTTATGACAAGGGAACTGCTGGGCTATAACATTGTTTTATGGTGAGAGCCCAA
TGCAATTTTAATGCACTGAGAAACCAAGCTTTGACTTTTTAAATGTACATTTGTAACGGTATTTAATAAACCTGTTTAACTCAAAAAAAAAAAAAAAAAA
AAAA
5' EST: CF785367
[+]taattaatcccccccccccccccgctacgtgtctccccagctgttgttgttaaatctgctattgtgctgaaccaattctacttcacatctttccccaccg
aggaaacgcaagcttatcgtatgtcagctatccagaacctccaacctttcgacccatttgctaatgctactacaggcggcatcttgctcccagctgggtc
acaggactccatccgtataagaaatacagcatatgaacggcaggaaaaccctcaccatggtacagggcataaccgatgcctatgataagaagatactagt
gaacgccttcaagaagaaatttgcctgcggtgggaccctgatgtaacccccagaatacggaaaggtggatccagctgccgggataacaacgcaataaatg
ccagtctgtttttgaaaccagtgtagggggtggagtaaggaaaaaactctattaaaaagggccttgtagattgcaaaatctcctgtattattcttggatt
ttagatacgttacatgactttctttagccctggtatgtggtgttaagaatttttcaataacggacttctctttttaatatatattataaagtatactttt
attttctatattgaacttagccatatttcaactatttaatccccacttctaactccatggcttagttgtttatatgtacatctatatctatatatgatga
tttagtcggattcttaaatgccggttatgacgcgagaccaaccaatacaatcctctatgtgagtgcttatgggggtctcaaatataacatagtcacatgg
atccctgggactcaccattctattttgtgcgtgctccaaaattgcgtatgtttttctccattgagatggaggagaacgaaaatagtaagttcgcatcacg
cattgatatgtcaagagttattctagataaagacctcagtgnccaacgcctaatataaaactatctcttgggaacgtctattccataacgaacagtcacc
attatttcgctctgcctataatactatggtactatatgttgtatattatctgtataaccatataaatcgacctctacataataatctccatcacgactca
tacan
3' EST: CF785366
[+]tttttttttttttttttttttgagttaaacaggtttattaaataccgttacaaatgtacatttaaaaagtcaaagcttggtttctcagtgcattaaaatt
gcattgggctctcaccataaaacaatgttatagcccagcagttcccttgtcataagtttaatactgaacagccacttcatactggacattactggggact
gaagtctcaatgggccacctcctgctgctttggccagcacaatattagccatctttactgtaagccataaccaggaaagcaagatttagcctcatcttac
ctccccagtctggtgtgtatagaagtcatacttagttacggtgggtctgtggcagcaatgggaaagcattgcccctgtaggggcaggcagctgtacagac
attctcaggcttataaccacacactgtgcgcccacaatttgtccataaaacagttatttctcatataaaggagtgactggagaataagcttgtgataagg
gagccggaagcaactacacactgcagcagggagccgtgtgttcaattccaaatactcagacgttaaaatccatggaccttcaactggtcttcctttgcca
gcccaacctcagttagaaactgacaggcatttttgcgctggtctccctgcagctggatcacctctccgtattctgggtgttcaaccaccgtaccattgca
agcaaattttcttttaaaagccctcactaattttcctcttaacataagcatcggctatgcccctggaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: