XB-CLONE-4350684
Clone Name: IMAGE:7025950
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785370 [+]
taattaatccccccccccccccatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagagaa
aggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcaggacagaga
cggcatcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcatct
ggccccatcaacttcaccgtcttcctgaccatgttcggcgagaagctcaagggtgctgatcccgaagacgttatcactggggcattcaaggtgcttgatc
ctgaggccaaaggatccatcaagaagcagttccttgaggaactccttaccacacagtgtgacagattcaccccagaggagatcaagaacatgtggacagc
gttcccccctgatgttgctggcaacgtagactacaagaacatctgctatgtcatcacccacggagaggacaaagaccaggaataaacaggcccagtcttg
ccctcccgatggaaggggtggcgccatcacgccccatcaacaacccttgctgaaaattggcgtctaattttataaactcctcccctccgaaaacacaccg
ggaatttatttcttccaagggggggttctgaaaaggggacccccccccggtggtccttggtccccttttctcccctgtcccccttcc
3' EST: CF785371 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttgaataaaaagttgactttattgttcttcccttgagggctccaaaa
ttccagtttaaaacatcaaaaagcgaaatgtgagggagggtaaaaaaggagggaaagggacagccagaaaggggacaagaacaacagaaaggggtcccct
ttcaaaccacccctaaaaaaaataaattacagttgtgtctctgatgggatgagtttataaaataagacgcaatatacaccaaggttggtgctgggcgtga
tggcccaccccctccatcaggagggcaaaactgggcctgtttattcctgggctttgtcctctccgggggggaagacataacaaatgttcttgtagtctac
gttgccagcaacatcaggggggaacgctgtccacatgttcttgatctcctctggggtgaatctgtcacactgtgtgggaaggagttcctcaaggaactgc
ttcttgatggatcctttggcctcaggatcaagcaccttgaatgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtca
ggaagacggtgaagttgatggggccagatgcctctttgaccatctcttccaactctttcattcttcacgttcaggcggcccatagctgcgaaatgggtcc
ctcaggtcttccttgtcaatgaagccgctctctgtccctgatcaatcacctgggaaaaccttcccttggaactccctagaatcttggggtttgggtcaaa
acatggggaccaacaccttttggaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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