XB-CLONE-4350674
Clone Name: IMAGE:7025946
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Gene:
hspa8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785376 [+]
taattaatccccccccccccccgctcattgcaagctttacaagctcggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactgagag
caacaatgtctaagggaccagcagttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaatga
ccaaggaaacaggaccacacctagttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccacaaac
acagtttttgatgccaagagactcattggccgcagatttgaagatgcagttgttcagtctgacatgaagcactggcctttcaatgttatcagtgacagtg
gaagaccaaaggtccaggtagaatataaagcagagaccaaatctttctatccagaggaaatatcatctatggtcttagttaagatgaaggaaatagctga
agcataccttggcaagactgtaacaaatgctgtaattactgtcccagcatattttaatgactcccagcgacaggctacaaaggatgctggtactatatct
gggctgaatgtactgcgaatcattaatgagccaactgctgctgccattgcttatggacttgataagaaggttggagcagaaagaaacgtgctgatctttg
atcttggtggtgggcacctttgatgtctccattctaaccattgaagatggcatcttttgagtaaagtctaccgn
3' EST: CF785377 [+]ccaaccaatacaatatcgtgtatttgtgacttgtcaagcttagcatcacgaagtgctttttccacagggtcaagggtacctctaaagagatcagcattaa
gttcttcaaaacgtgctctggtgattgaggtatagaagtcgattccttcatagagagaatcaatttcaatgctagcctgagtgctggaggagagcgtacg
ctttgcacgctcacaagcagtacggagacggcgaacagctctcttgttgtcaatgatgtccttcttgtgctttctcttgaattctgcaacaaagtgattc
accatacgattatcaaagtcttcaccacccaaatgagtgtcaccagcggtagactttacttcaaagatgccatcttcaatggttagaatggagacatcaa
aggtgccaccaccaagatcaaagatcagcacgtttctttctgctccaaccttcttatcaagtccataagcaatggcagcagcagttggctcattaatgat
tcgcagtacattcagcccagatatagtaccagcatcctttgtagcctgtcgctgggagtcattaaaatatgctgggacagtaattacagcatttgttaca
gtcttgccaaggtatgcttcagctatttccttcatncttactaagaccatagatgatatttcctctggatagaaagatttggtctctgctttatattcta
cctggacctttggtcntccactgtcactgataacatttgaanggccagtgcttcatgtcagactgaaacactgcatcttcaaatctgcggncaatgagtc
tcttggcatcaaaaactgtggttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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