XB-CLONE-4350665
Clone Name: IMAGE:7025942
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785382
[+]
taattaatcccccccccccccggcatctttgcactctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcctata
ggtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttggagg
aggctttggcagcggtgcaggctccggcttctcatcctctgggggtaactttgccatggcggcggcagacagttccaacttaagcaacttcggtggaaat
gaaaagcacgccatgcagaacctcaacgaccgtcttgcttcctacctggacaaagtaaaagccctggaagcctccaatgtcaccctggaagggaagatcc
gtgactggtatgatagcaaagtggatgccggccttggagctgggtctaaagattacagcaaatattttgagatcatcaatgagctgaggagcaagattgg
tgccgcaactgttggcaacgctacagtgaccctgcaaattgacaatgccagactggctgctgacgacttcagacttaaagtttgagaatgagctggctct
tcgccagagcgtggaggctgatatcgctggcctgcgtagagtcctggatgaactgaccctggctagaggtgacctggagatgcagatcgagagcttgact
gaagagctgggcttacctaagaaagaccatgaggaggagatgagtcatgcaaagaagccagagcgcccggcaaaatgagtgtttgaaa
3' EST: CF785383
[+]tttttttttttttttagagttaaaatcttttattgctttatgagaaacgtctataataatttcttcttttcttagaatctaagggaacattcctgttcat
ttgtgcctttatctttcattgccttggttttattcaattctggatgaaacaaccttcccatctaccacctcctcgacaatggttttcaccattgtggttc
tagtggttgatgattctgttttggactccacggaacttgccttggccacggtggtcatctgtcccagttccccttccagcaggcgcctgtatgtttcaat
ctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccatgtcggacctgatctgcagaagctgctcctctaggctg
ctgatagtgttctggatctgtaaaagttgcgctgaatagaagccttgtacttcactgangttgccttcaactgattgtttcattgccagcagggattgca
gctcaatttccaagctctgaagtgaccgtttcagctcggtgatttcgctcttgctggcctgaacttgttcaaccccaactgaaatctctttcttaacctc
accactcttctggttgaaccacaactctgcatccctgcggttcttctcagccagtatttcatagtcagctctcatgttattcaaaatgttggtcagatcc
acgcctggagccgcgtccatctcaacactctctttgcgggcgctctggctctttgcatgactcatctccctctcatggttcttcttccggtaagcccacc
tccttcatcaagcta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: