XB-CLONE-4350645
Clone Name: IMAGE:7025934
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Gene:
ocm4.5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082747 [+]
GAGAAGTGTTAATACTCAAAAGGTTGCTCAGAGCCCTTTGCTCCTGTTAAAGAGGAATTCAGGTTTACCATGGCATTCGGTGGAATCCTGAGTGAGGCTG
ACATCTCTGCTGCCCTGCAGAACTGCCAAGCCCCTGACTCCTTCAACTTCAAAACTTTCTTTGCCCAGTCTGGCCTGAGCAGCAGGTCTGCAGATGATGT
GAAGAATGTCTTTGGCATCCTGGACCAGGACAGGAGTGGCTTCATTGAGGAAGATGAACTAAAACTGTTCCTCCAGAACTTCAGCGCAGGTGCCAGGGCT
TTAACTGATGCTGAGACCAAGGCCTTCCTGGCAGCTGGAGACTCTGATGGTGATGGCAAAATTGGAGTTGAAGAATTCCAGGCTCTAGTCAGACCTTGAA
AATGAAGACCACAAACTTTCATGAAAGATGAAGTTGCAGGAATTTTGGCTTTTCTTAGTTAAATTGAAAGAAGTGGAACCTGGTTGGGCAGAATGCAGAA
ACCTATGGACAAGCAAAGGAAGGGCTTTGTGCTGCATAGAAGAAACGTTGTCGGGAAACCAGCAATTTATCTAAGAGTTGCCTAATTAACAGTATTTATT
TAACATGTTATATACCACTGGTCTCTGATCATATCAGCATGACTTTTGTAAATTAGCATGTGATTCTTCTTTCAGACTGAGGCAGCACCATAAAAAACTC
AAAGCTGTCAATAAAAACAATTTTTGTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF785394 [+]taattaattcccccccccccccgagaagtgttaatactcaaaaggttgctcagagccctttgctcctgttaaagaggaattcaggtttaccatggcattc
ggtggaatcctgagtgaggctgacatctctgctgccctgcagaactgccaagcccctgactccttcaacttcaaaactttctttgcccagtctggcctga
gcagcaggtctgcagatgatgtgaagaatgtctttggcatcctggaccaggacaggagtggcttcattgaggaagatgaactaaaactgttcctccagaa
cttcagcgcaggtgccagggctttaactgatgctgagaccaaggccttcctggcagctggagactctgatggtgatggcaaaattggagttgaagaattc
caggctctagtcagaccttgaaaatgaagaccacaaactttcatgaaagatgaagttgcaggaattttggcttttcttagttaaattgaaagaagtggaa
cctggttgggcagaatgcagaaacctatggacaagcaaaggaagggctttgtgctgcataaaagaaacgttgtcgggaaaccagcaatttatctaagagt
tgcctaattaacagtatttatttaaaatgttatataccacgggtctctgatcatatcagcatgacttttgtaaattagcatgtgattcttctttcagact
ggaggagccccctaaaaaactctaagctgttcattaaaaacaatttttggtaatttaacgccgacagaaggcatagaagagacat
3' EST: CF785395 [+]tttttttttttttttttttttttttttttttttttttttttaaaatacaaaaattgtttttattgacagctttgagttttttatggggctgcctcagtct
gaaagaagaatcacatgctaatttacaaaagtcatgctgatatgatcagagaccagtggtatataacatgttaaataaatactgttaattaggcaactct
taaataaattgctggtttcccgacaacgtttcttctatgcagcacaaagcccttcctttgcttgtccataggtttctgcattctgcccaaccaggttcca
cttctttcaatttaactaaaaaaagccaaaattcctgcaacttcatctttcatgaaagtttgtggtcttcattttcaaggtctgactagagcctggaatt
cttcaactccaattttgccatcaccatcagagtctccagctgccaggaaggccttggtctcagcatcagttaaagccctggcacctgcgctgaagttctg
gaggaacagttttagttcatcttcctcaatgaagccactcctgtcctggtccaggatgccaaagacattcttcacatcatctgcagacctgctgctcagg
ccagactgggcaaagaaagttttgaaattgaaggagtcaggggcttggcagttctgcagggcaacaaaaatgtcaccctcactcaagattccaccgaatg
ccctgggaaaacctgaaattccttctttaacggagcaaaaggctctgaaacaaccttttggagtaattaacacttcctcggggggggggggggggaatta
aattaacctccggggggaaaaaaaaaacaaattaactttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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