XB-CLONE-4350639
Clone Name: IMAGE:7025932
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Gene:
nop2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785398 [+]
taattaatcccccccccccccagccagcctgggcaacacgtgagaccgagtagaaagcgactccggcagtaggtgcatcatgggtaggaaattagacccg
ctgaggaaggtgcctcgtggcccaggccgtaaagcaaggaagcagcatggggcagaaaatgagctaaagaacatcctgcaagaggacacagaagcaaagc
gcatgtccagccgcggcaggaagagggcactaaaaaggcgcatgttacaggagcaaaaagcagcagccaaacccccagcaaagagcaatgccaatgcaaa
gctcaaaataggcgaaaagaaagtcctgccgggatttactgatgacaatgcagaatggctgaagcctgtgaaaagagctcgcaaggaagaagatgaggaa
agcgaacatgatagtgaggaggataatgaacaaaaagaagagggggatgaaatggaggaagtagaaaaagggatggaggaggaagtagatgaaaaaatgg
aatagtagaatgatgagaggggaagatgcctaataagtaagtgtaaatgaagatgggtgatgataatgattaagaaggaagaggtaggttgatgaacgaa
ggaagaattggttactatcgactaaattattataacagttctaggtcgtattgttggtctacacgcgtatagtaatacaaatacgtattaaatgcactcc
acaatatatattcacttgtaataactttatgatcgtatatcgtttacagagagtatgtgcccttgtcctatcgacctacgttcttcaatacttttttaca
tctacttagatactgctacgtatcatn
3' EST: CF785399 [+]ttttttttttttttctccttcttggatgcccgctctattggaagaagatccttctcatcccctgacgaggttccataatcatcctgaagatcatcgctgt
ctgacagggagagggattcgccaaactcaccatcaccatcctcctcctcatcaccatcctcctcctcctcatcatcatcatcaccatcctcatcttcact
ttcctcttctgcatcttccctctcatcactcttctcttccatttcttcatctacctcctcctccatcccattctctacctcctccatttcatccccctct
tcttcttcttcattatcctcctcactatcatcttcgctttcctcatcttcttccttgcgagctcttttcacaggcttcagccattctgcattgtcatcag
taaatcccggcaggactttcttttcgcctattttgagctttgcattggcattgctctttgctgggggtttggctgctgctttttgctcctgtaacatgcg
cctttttagtgccctcttcctgccgcggctggacatgcgctttgcttctgtgtcctcttgcaggatgttctttacctcattttctgccccatgctgcttc
cttggtttacggcctgggccacaaagcacctttcctcagcgggtctaaattcctacccattgatgcaccctactggccgggattccgctttccaactccg
ggtctcaacctggttgccccaaaactttgtctgggggggggggggggggaatttaattttaacttccggggtggcaaaaaaagaaaccaaattagccttt
ggtatttttcgggggccctccggataaaaaaaacccaagaggtgcccaaatgtgggggaacagcga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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