XB-CLONE-4350633
Clone Name: IMAGE:7025930
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Gene:
psmd10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785402
[+]
taattaatcccccccccccccgggaggaggtgagggtgtctgatgtggaggtgtgtaacctggcttacaggggcgacctggaggagctgaaaagtcgcat
ccttgccgacaagtcgctagccacaaagacagatcaggatgacaggactgctgtgcactgggcttgttcagcagggcgcacagaggttgcagaatacttg
ctgagattaggggtgcctgttgatgccaaagatgatgcaggatggactcctttgcacatcgcagcatctgctggccgcgatgatatcgtgagagcgctaa
ttgggaaaggggcccaggttaatgcagcaaatcagattggctgtactccattacactatgcagcatccaaaaacaaacatgagattgctttaatgctgct
ggaaaatggagcctctcctgatgcacaggataatttggagtcaactccactgcacagagcggcttctataggacaccttagaatcgttcagattcttctc
aagcaccaagcttcaactcatattcaggatacggaaggaaaccaccactccaccttgcctgtgatgacgagagagtataaaaagcccactgtctggctga
acctggatcaagtctttattttgcgaaaccgggaatacaaaacccccttacaagttcgccaaggggggccccttgaccctttgtctgggggaaaatcctt
ataaaaggcgtgcttttcctaattacctttgggttgctatccggggccttcctggcgcgcctccaaattttattccccgcttatcctggtcagccacccc
cgctctgggccctcggcgatcaaaagttc
3' EST: CF785403
[+]tttttttttttttttggtccatagacatttattgaaataaacagcattcagcccttttgtaacagcgtagtgtaatgcatttattggatcattttggatt
atttgcttgggtcccataaactgctacttcatactcggagaaaccatgctttggaaatgacatgagtgttctggcaacaaactttatttgagcaacttta
acctctagaatgaaactttgcatccttgttctgacttttgtaaactttgctcttggtgaacctctttaaaccaaaggtcgccacttctacaaggcataag
aggtgctacatatactgggacaaagctaggccacagatgcacaataacaacaaagtactgaaaaactacccttctacgattctcctcaacaaagatccta
agcccccccttgcaacttgtaatggtgttttctcttccttgttctcaatataaatacttgctccatgttcaaccaaacacttggcttcttctactctctc
ctcatcacangcaaggtggagtggtgtgtttccttccgtatcctgaatattagttgaagcttggtgcttgagaaggatctgaacgattctaaggtttcct
ttagaagcccctctgtgcagtggagttgactcccaattatcctttgcatcaggagaagctccatttttcagcaacattaaagcaatcccatgtttgtttt
tggatgcggccaaatgtaatggagtacagcccaatctgaattgctgcattaaccgggggccccctttcccaatttacgctcctcccaaaattatccccgg
cccagcaaaatgtctgcaattgccaaaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: