XB-CLONE-4350628
Clone Name: IMAGE:7025926
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785405
[+]
taattaatcccccccccccccgctatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagag
aaaggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagattccggagttcaaggaggctttcacagtgattgatcagaataga
gacggtttcattgaccaggaagacctgatggatacctcctccactatgtgccgcctgacattgaataatgaagatttttaaaaacatgtctattaaaaat
tggcgttttcaagcttactgctttcacgacctggttcgttaaaattttaggagtcagaacttccgtagtctttttcccaaaacagtcaaacattttaaaa
tctagctccgcctttggaattccgtcagactcatgtccctttccattgcaccccctttaaaacccctattttttgacaaagtttgcttacccatcgttta
ttaccttgcccaccgggtgcggaaattttttaaaaaccccctcatttagattttcgtttggtgttccacatcccatttttttcacaattattactccacc
cgttctatttttttcgtcagtaatttccccaacttctataatattcaacataccccccttaatacctctccttatttccgtatcccacgtatccgcgtcc
ccattatttatacccaccccctatttatctcttttcccgcacgtcccacatcgcgtttctcccccgcttatttgtcccatggtgagcgacctataccgta
tagtatgcggtctacgacttcgccattatatacggtctcacatatctttcgtttccaaaaagctgccgcgattttcacaagaaagtacggggcctcgcga
tataactacatctatctagtgtttttgttgtcccaagtgtctgttttctgtacgccgtcttcacaatatatctttactgtcgttagtttagctataagag
agttgcggtgcagcgatgataattgtctatgtcaccttg
3' EST: CF785406
[+]tttttttttttttttggataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagggaggg
taaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttgtgtct
ctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactgggcctgt
ttattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccacatgttc
ttgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcaagatcaagcaccttga
atgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtcaggaagaaggtgaaatttgatggggccagatgccctctttg
accattctctttccaactcttcatttcttcacgtttcaagccgggcccataacctgccaaatgtgtcccctcaggggcctccctttggtcaatgaaggcc
cggccccgggttccggatccaaccaccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: