XB-CLONE-4350622
Clone Name: IMAGE:7025924
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Gene:
prpf39.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785409 [+]
taattaatcccccccccccccggcggaggcgatcacgcatgcttcgttgtggcacgattaggggctgagagcggccttcagtttcctgccgctttttttt
atagcgacggtctcttttaggggacttgtgggagatactattaggaatgaccacttcaagcaatatccaaaactcatctatgctagcttcaaaacatact
tgtgtcgaggaagtagattttactaaagacattgtttaagtgttggagatggagcagtctcctttactctgtgcaacagaattacctctctgtgcaacag
aacataaattgccaacaacagaatcgtttttgctaaaacaacatacaccttgctctaactacttcctccctgatttttgaaacacgatgggttaatcgtg
atacatgcgttatccggggattgacaattaaatgtcttcggggctatcctgtgtcagacttgtgatagttgccatccaaggaagaaaatttattcgcgct
ctcggccttgtcctggggaagacgccttattcttcccgccctttaacggctgcccagggttcattcaccccattctgcgccttaatatggccccccgcgg
gtacctgtagacaaattggtaaagaacttgtggggaagcagaaacacggatatttttttgttttgcgggttcttttcgcgcaaacgaacatcgtatagta
aagcgtggccgctgtccttggtggataacaatgtgggggagccaattaagcacagaggggggggcggcgacagtacatacaatgtggttataacgtttgg
gccgggccggagacaatacttctatagtgaggagagcgcgcacgtctatttatgtnccggncgattcaaagagatcctcccccggcatatatgtgggaga
cagacctgtgtatataaggttggtactatgttgctggccgaggtactcgcgccacaagggcgaacactgctcggtgccttatatcctctgcgtcggctat
aacgtctatgcgtgaagaaatntatatatttcgtatatatcttttgtgagaaggagggttgggcggccnagntcctgtaattcgtgaagggagggagacc
gcatctacacattaataattcgtctctnatatcgggagttctttcttttccatggttagaagagagcatattttgtttcttgatgtatctcgggtgacnc
caaacccgctccatatgttgg
3' EST: CF785410 [+]ttttttttttttttggggtgggcaactctttattgataaaccaaataacgcttgaggagaatgagtaaacattacacagatgaatgcaggcaacttcaat
tctaaaatcacaatgcttttttttttttttaaaaaaaaaagagaaaaaaacccaatactgatttaactattgaaattaaatttgtctattctaacagatc
tactaaaagtaaagttaagcagaatgccttttcatataaatgtgcactttctttcaaagtgctagactgtgctaaacacgtatttgttgggcatattttt
ttaggctgttccattagacactgatgctaagcaacaacagtttatccaaactacaaaagattcaggatattagaaaagaaaaacaaaaaattatgaatag
atgtggagtaatacatttccaatggacacacatttcagccccaacacaaaaaaaaactgatacacttatatattccatggattccaggaaaaaaattgaa
acatgaaaaaaaatgtttactgtgctcactaagtgtgcggctgcagtgatggtaaccagtgcccaggttctcccaatttggaagaaataaaaaggacatt
tcacaacttgaaaagaaaaaaaaatactcaatggtttacaggagtattcatagtgctggtttagtcttcaagttgatggagtattgtagtactgtccata
attccagtttggcaaggtagttgtactgataccagctccccgtagttataacctgactgagctgcatctacacttgcagtggtaactggaactggagcta
ctgtgctcacttcttctgtatgcagtccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: