XB-CLONE-4350605
Clone Name: IMAGE:7025916
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Gene:
actb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785420
[+]
taattaatcccccccccccccgtcagtgacccgcccgcacagaaacaggacagtgtgtgtgtcgtactctcagattacaatggacgacgatattgctgca
ctggtcgttgacaatggttctggcatgtgcaaagccggctttgcaggtgatgatgcaccccgtgctgttttcccatccattgttggtcgcccaagacatc
agggtgtcatggttggaatgggacagaaagatagccacttatgagacgaagctcaaagcacaagaggtattcttaccctcaaataccctattgagcatgg
cgttgtcactaactgggatgatatggaaaagatccggcaccccaccttctttaatgaactctgagttgccatgtaggaaaaccttctgctgccgccccaa
ccacccttgaatcctaaaagtgacctgggagagaacgacgcatattttggtttacattcacccttttaacccccgcccccatgttttgttggcacattca
tacgttgtccattgcccccctgttaactccattttacggattatgatgtacccgtcgaaatttggctctctcaaaaccaccatgagactgaatggggggg
ttgttcttcgtatcttcccgtaagacccacacaccccggactttattattattgtatattgtacgtaaacagagcccgaccaataatgtcgccagaatgt
ctcggggcatctttcacttacggagtgaccaagatgactcgttgtgtgtatataagaagagtgaacccacgcctgtggtggcatcacataccgctaggaa
cagcccccacgcnaagggggcacgacgtacacggacatctaacttccgttctcagagagctgcggtggagtntgtcaagtcatagaacgtgacgccgccg
gcacgatctaatactaatagcggtccgactaaccccgtcctctactatatattgtgacgttatagcgcactcgtgcggtgtttaagcctgcatgggcgtg
ccgcgcaatattttttcgctgcaccccgatataatagtatatctcgctatatttctccacgtcgcccctcgtctgtgttgttctcgtgcagctttagtgc
gcgtttataccccgtcgacggcagagtaagaacagataagttctcattggacgcggttctatgtttaggttaaagaatatggaaagatacgtcaacgatg
gtcctatt
3' EST: CF785421
[+]tttttttttttttttgaaacaacttggttttatttttttcagttaggttacaggtgacccgtcacatcttatgtgatcaatgataatgtaaaccttccag
gtgttagagaatgatgcccttttttgggttaaacaagttacaagtgttcaaggcagaagatacagagacatgtttgggtacatagctgcacaagaaaaaa
aaacaaaaaaaaaaaaaaaaaatcaaaaaacacaatagtatcaccatgatttctgtatgagcttcccaccaagctgggcaacattgaaagggtagcttcc
tcaatacaatactattgactgaataaaatcataatgtcctctgcattgatgaccatacagtgctatttgttcattccagttatcaaatctgagccaagcg
ccaatgaaagaaaagccatgccaatgttatcttcctgcacaggtacatttgttcacgttgaaagggtccgtccttttagaagcatttacgatgcactatt
gagggcccagactcatcatactcctgcttgctgatccacatctgctggaaggtggacagggaagccaagatggaaccgccaatccagacagagtatttac
gctcagggggagcaattatcttgattttcatggtgctgggagctagtgcagttatttctttctgcattctatcagcaattccaggggtacattgtggttc
caccagagagaacagtatttggcatagaggtccttacggatatctacatcacactttcataattgaattttgtaatgggtttcctgaatgccctcaggat
tc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: