XB-CLONE-4350600
Clone Name: IMAGE:7025914
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Gene:
fbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785423
[+]
taattaatcccccccccccccggctgactgctgacctgtccatctagcagtgtacttacctgtccagagccactaacagcaccaccatgacggaccaggc
ccccttcaacaccgatgttattaccctgactcgcttcgttatggaggagggcaggaaagccaaggggaccggagaattaactcaactccttaactcgatg
tgcacggctataaaagcaatctcttccgcagtgcgcaaagctggcctggccaacctctatggtattgccggatccactaatgtgacaggggaccaagtga
aaaaactcgatgtcctctccaatgaccttgttatcaacatgctcacagtccccatacattgtctgtgtcctagggtcagaagaagaccatcatgctctga
ctgtggagcctgaaaaaaaggggtaaatacctggtgtggctttgaacccctggataggctcttcctaaccgtttaaccgccccgcctttttttttggtta
aaacaattttttcctattctcaccaaaaaacgattttctttaatgggaccaatctcaatcttgcacaaaaaaaattgccccccactaaaccatatgtatc
gaaataacatttggtataggtgtggacaagagactaattgaccccccctaaattttttggcgacggtgggccagaaacagaggtgggggttttgggggtc
attctttaatggcccccacactaattctaaatcttttattgcgggtgggcggccacacaaaaatgtgtagtagatttacccacggagtttttcgggtgag
acccccataggagccaaaattttgggggataacgagcgtttctcacattttcttttttaacgggaccattacgtctatatgaaaaactgactgtcgacca
cgccgactctaaatataaaaatatcgatcgcgggttccccacgcccttttgtgtatatcttgcccttctggctatttgtcataggtggctccacaccaat
ttttatatagtttcaccatcctctgggcctccgacatttacacttctcgccgcgcggatatttacttgtagtaatgggagaaagcggctcagtctttttc
ctaaaaaaatagttcgcggtgggtccattgaaaccttaaaggcggcgcacactcaccttt
3' EST: CF785424
[+]tttttttttttttttataaaaatctatttgttattgtgtacaaaagtaaacatatttctttatgttttggcacattaaaaagaagctgctggtttattct
ttggtccttcttgatatgaaatccattcagctgtcctttatttctttgcatatttcttgtaaatgttaatatattcctgcacatcatctggggagcccaa
agctactggcatcctctggtggatactctcaggtactacatcaagaactgactcaagaccattagtagccattcctccggccttctccataatgaaagcc
attgggttgcactcatacagaagcctcagcttgccctttgggctctttacattggctgggtatagaaagattcctccataaaccaatgtacgatgcacat
cagctaccattgaaccaacataccttgcaccgtagggtgagctcccatcttctggaaatttctttttctgtaagtattcagtaactgcttcatcagaata
cttggcataaccctcattcaggctgtaaatgttgcccttctttttgattttcacatcctggtttactaaaatgaactctccaattgctgggtccaacatg
aaacagttgacaccacattctgtggcaagaaccaacattgttgcactgccatatanggcatagcctgcagcaacaatgtttcgnccctggttgtagagca
tctttttcagttggctctccttctgtagtcttttttgtagatggaaaaatattgtaccaatagaaagccaagcagtcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: