XB-CLONE-4350597
Clone Name: IMAGE:7025912
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Gene:
cdk11b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785425
[+]
taattaatcccccccccccccgcgcaaagaacgagacctcaaaagagaagcagctgcacatcacagaacagccagggaagagcatggtgaaaaaaacaaa
gcagctattcgtagtcgaagtcccataaggcagcaacgagagagattagttgcaggagatgttaagaagcaaggaaaagatgaaaggctagacacacgaa
caattttttcagatttgcaagatgtcagtgacagtgaaaggaaaacatcttctgctgaatcttcctcagcaggtactgggtctgcttcagaggaggaaga
ggaatccagtactgaagaatctgaggaaggagaaaaggaggaggaggaggaggaagaagaagaagaagaggaggatgatgatgatgaagaggaggaagaa
acagggagcaattcagaagcagtatctgaacaaacagctgaagaggtcagtgatgaagagatgagcgaagaatgagagggagatgggaatcatngttcca
tagaatccagatttgaccatgactcagcagagagccgagaagaagaagaaggatgaggngacattagaggagtttgtccacattcaattcaccaacggat
gggggataacgtccagactcttcagtcatgtcaccgggtgagctaaagcagaacttgccaaatacctttcaggcccttcatgtgggaaaaattccantgc
ttgaatccnattnaanaaaggacnctttgggggttattgtaccaaaccgcaaaaatcttaaaactggagaaaattttggggcctcaaaacggcctggaaa
ag
3' EST: CF785426
[+]tttttttttttttttccattttcagccgcttcagagccacaatttcatcagttttacgatctttcgctctgtacactacaccatacgtcccttcttcaat
tcgattcaagcactggaattcttccacactgaagggctggaaggtattttggcagttcttgctttagctcaaccggtgacatgactggagagtctggaac
gtattccccatctgtttgtggatttgaatgtggactaacttctcttatgtcttcttcatcctcttcttcttcctcgctctctgctgagtcatggtcaaat
ctggattctatgggaacatgattcccattctccctctcatcttcgctcatctcttcatcactgacctcctcagctgtttgttcagatactgcttctgaat
tgctccctgtttcttcctcctcttcatcatcatcatcctcctcttcttcttcttcttcctcctcctcctcctcctcttctccttcctcagattcttcagt
actggattcctcttcctcctctgaagcagacccagtacctgctgaggaagattcagcagaagatgttttcctttcactgtcactgacatcttgcaaatct
gaaaaaattgttcgtgtgtctagcctttcatcttttccttgcttcttaacatctcctgcaactaatctctctcgttgctgccttatgggacttcgactac
gaatagctgctttgtttttttcaccatgctcttccctggctgttctgtgatgtgcagctgcttcctcttttgaggtcccgtctttccn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: