XB-CLONE-4350586
Clone Name: IMAGE:7025907
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Gene:
selenof
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785433
[+]
taattaatcccccccccccccgaccagtgcctagtgggaagatggcggcagagaggatgctgctgtggttagtgactgtgctacaggcgctgtcgtctta
tggtgcagaactctcttcagaagcatgcagagatcttggcttctctagcaatcttttgtgcacttcttgtgaattgcttggacagtttggtctgagtgag
atcaacttattctgcaggcagtgctgccaggaagaagtacacctagagtctaaaaagagatatccaggtgctgtacttgaagtctgtggatgaaaattgg
gaaggtttcctcaagtccaggcttttgtcaggagtgagaaacccaaacttttcaagggtctgcagatcaagtatgtacgtggctctgaccctgtcctaaa
gcttctggatgaaaatgggaacatatctgaagaactgagcatactgaaatggaatacagatagtgtggaggaatttttaagtgaaaaactagatagatta
taaagagttttttctatttttagcctttaatttgaaaagtttcatgtacaaattctgcttctgaggtcataacatctagcattattttgcatttataaaa
ttcattggcagcattccatttttaagtgcataaccctctgagtggcctagccagtggtaaagatttactactgcagctgtctgtgtactgaaagaaagtt
ttttgctagtggaatagaaaccggctctttccagtgggaagcattncttacccctaataactttaaagaatcaaaaaaaccttttttaaagtaaccccaa
atattgtaagggttta
3' EST: CF785432
[+]gcgtttgcaagacacatttagaaaaatccagaaatacaaaaatgcaaatggaagcatttatgacaccaaaagacagggtaaaattcaaataagttttttt
ttttttttttgaaagaaagaatccaatcaaatctttggctttagacccagtatacagccctcctggccccccttccaactgcagctatagcagctaccat
gttggtgaaagcagcgagtgcagcaactaggaatggggacactgatccatcttagccacgagtgtgcatacaaattgggtgagcaaacattgtgcacaca
gtattttgagtgaaaataatttagtacttattgtgagtagcccacttctcagcacaaactgctctgtttttaaagcacacaaaaatagcaaaaaggaaat
tcgaggaaatagtgattaaggaatgttcactgggactagaggcaaaagcttcagtggtgtttgctttccctttaaacacatttaaggcttctaaaattgt
acagaagtgtaacaaagagaaaaagtctgtttataaacaatgttgaaataacttactgaatccaccctgtgtttatggtatggtgcacggaaaacttcat
ttctgaaatgcacaatataaatgataatctaatggcaatggtgcaagtttacagactacgtttggcatcctcatcctaaaattgcccaacatattttggt
atacatgtcaaaaagtttcatttttcaatgagggtcagatagtttgtgcacattacattttgtctcagctaaaggttttctagcattcccttta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: